First derivation in Spain of human embryonic stem cell lines:: use of long-term cryopreserved embryos and animal-free conditions

被引：50

作者：

Simón, C

Escobedo, C

Valbuena, D

Genbacev, O

Galan, A

Krtolica, A

Asensi, A

Sánchez, E

Esplugues, J

Fisher, S

Pellicer, A

机构：

[1] Ctr Super Alta Tecnol, Valencia Stem Cell Bank, Valencia, Spain

[2] Fdn Inst Valenciano Infertilidad, Valencia, Spain

[3] Univ Valencia, Sch Med, Dept Pediat Obstet & Gynecol, Valencia, Spain

[4] Univ Calif San Francisco, Dept Stomatol, San Francisco, CA 94143 USA

[5] Univ Valencia, Dept Pharmacol, Sch Med, Valencia, Spain

来源：

FERTILITY AND STERILITY | 2005年 / 83卷 / 01期

关键词：

D O I：

10.1016/j.fertnstert.2004.09.004

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

The first two human embryonic stem cell lines (VAL-1 and VAL-2) have been derived in Spain with long-term cryopreserved embryos under animal-free conditions. In the first series, 40 human embryos that had been cryopreserved at day 2 of development were thawed after >5 years. A derivation efficiency of 5% per frozen embryo or 12.5% per blastocyst was obtained (Fertil Steril(R) 2005;83:246-9. (C)2005 by American Society for Reproductive Medicine.).

引用

页码：246 / 249

页数：4

共 6 条

[1] ISOLATION AND CULTURE OF INNER CELL MASS CELLS FROM HUMAN BLASTOCYSTS [J].