Directed termination PCR: a one-step approach to mutation detection

被引：11

作者：

Chen, JJ ^{[1
]}

Hebert, PDN ^{[1
]}

机构：

[1] Univ Guelph, Dept Zool, Guelph, ON N1G 2W1, Canada

来源：

NUCLEIC ACIDS RESEARCH | 1998年 / 26卷 / 06期

基金：

加拿大自然科学与工程研究理事会;

关键词：

D O I：

10.1093/nar/26.6.1546

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe a novel PCR-based method that allows the generation of nested termination fragments by integrating both selective DNA amplification and directed chain termination into a single PCR reaction. These termination fragments can be examined for sequence variation in either denaturing or nondenaturing polyacrylamide gels. This method provides a one-step and highly effective approach for the detection of both insertions/deletions and single base pair substitutions in sequences up to 1 kb in length.

引用

页码：1546 / 1547

页数：2