Targeted ablation of the murine involucrin gene

被引：70

作者：

Djian, P

Easley, K

Green, H

机构：

[1] Univ Paris 05, CNRS, UPR 2228, F-75270 Paris 06, France

[2] Harvard Univ, Sch Med, Dept Cell Biol, Boston, MA 02115 USA

来源：

JOURNAL OF CELL BIOLOGY | 2000年 / 151卷 / 02期

关键词：

embryonic stem cells; envelopes; skin morphology; skin resistance; involucrin;

D O I：

10.1083/jcb.151.2.381

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Involucrin is synthesized in abundance during terminal differentiation of keratinocytes. Involucrin is a substrate for transglutaminase and one of the precursors of the cross-linked envelopes present in the corneocytes of the epidermis and other stratified squamous epithelia. These envelopes make an important contribution to the physical resistance of the epidermis. We have generated mice lacking involucrin from embryonic stem cells whose involucrin gene had been ablated by homologous recombination. These mice developed normally, possessed apparently normal epidermis and hair follicles, and made cornified envelopes that could not be distinguished from those of wild-type mice. No compensatory increase of mRNA for other envelope precursors was observed.

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页码：381 / 387

页数：7

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