Real-time PCR method to detect Enterococcus faecalis in water

被引：46

作者：

Santo Domingo, JW ^{[1
]}

Siefring, SC ^{[1
]}

Haugland, RA ^{[1
]}

机构：

[1] US EPA, Natl Exposure Res Lab, Microbial Exposure Res Branch, Cincinnati, OH 45268 USA

来源：

BIOTECHNOLOGY LETTERS | 2003年 / 25卷 / 03期

关键词：

Enterococcus faecalis; fecal enterococci; real-time PCR;

D O I：

10.1023/A:1022303118122

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A 16S rDNA real-time PCR method was developed to detect Enterococcus faecalis in water samples. The dynamic range for cell detection spanned five logs and the detection limit was determined to be 6 cfu/reaction. The assay was capable of detecting E. faecalis cells added to biofilms from a simulator of a water distribution system and in freshwater samples. Nucleic acid extraction was not required, permitting the detection of E. faecalis cells in less than 3 h.

引用

页码：261 / 265

页数：5

共 15 条

[1] Infectious disease - PCR detection of bacteria in seven minutes [J].

Belgrader, P ;

Benett, W ;

Hadley, D ;

Richards, J ;

Stratton, P ;

Mariella, R ;

Milanovich, F .

SCIENCE, 1999, 284 (5413) :449-450

[2] Rapid detection of Listeria monocytogenes in dairy samples utilizing a PCR-based fluorogenic 5′ nuclease assay [J].

Cox, T ;

Frazier, C ;

Tuttle, J ;

Flood, S ;

Yagi, L ;

Yamashiro, CT ;

Behari, R ;

Paszko, C ;

Cano, RJ .

JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 1998, 21 (03) :167-174

[3] ENTEROCOCCAL AND STREPTOCOCCAL SPECIES ISOLATED FROM FECES OF CALVES, YOUNG CATTLE AND DAIRY-COWS [J].

DEVRIESE, LA ;

LAURIER, L ;

DEHERDT, P ;

HAESEBROUCK, F .

JOURNAL OF APPLIED BACTERIOLOGY, 1992, 72 (01) :29-31

[4]

DUFOUR A, 1986, 440584002 EPA OFF RE

[5] Quantitative measurement of Stachybotrys chartarum conidia using real time detection of PCR products with the TaqMan™ fluorogenic probe system [J].

Haugland, RA ;

Vesper, SJ ;

Wymer, LJ .

MOLECULAR AND CELLULAR PROBES, 1999, 13 (05) :329-340

[6] The RDP (Ribosomal Database Project) continues [J].

Maidak, BL ;

Cole, JR ;

Lilburn, TG ;

Parker, CT ;

Saxman, PR ;

Stredwick, JM ;

Garrity, GM ;

Li, B ;

Olsen, GJ ;

Pramanik, S ;

Schmidt, TM ;

Tiedje, JM .

NUCLEIC ACIDS RESEARCH, 2000, 28 (01) :173-174

[7]

Messer JW, 1998, APPL ENVIRON MICROB, V64, P678

[8]

Nelson RRS, 2000, J CLIN MICROBIOL, V38, P2112

[9]

Oberst RD, 1998, APPL ENVIRON MICROB, V64, P3389

[10] Determination of 16S rRNA sequences of enterococci and application to species identification of nonmotile Enterococcus gallinarum isolates [J].

Patel, R ;

Piper, KE ;

Rouse, MS ;

Steckelberg, JM ;

Uhl, JR ;

Kohner, P ;

Hopkins, MK ;

Cockerill, FR ;

Kline, BC .

JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (11) :3399-3407

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