Optical imaging of in vivo gene expression:: a critical assessment of the methodology and associated technologies

被引：28

作者：

Golzio, M ^{[1
]}

Rols, MP ^{[1
]}

Gabriel, B ^{[1
]}

Teissié, J ^{[1
]}

机构：

[1] CNRS, UMR 5089, IPBS, F-31077 Toulouse, France

来源：

GENE THERAPY | 2004年 / 11卷 / Suppl 1期

关键词：

in vivo optical imaging; fluorescence; luminescence; gene expression;

D O I：

10.1038/sj.gt.3302374

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Following and quantifying the expression of reporter gene expression in vivo is very important to monitor the expression of therapeutic genes in targeted tissues in disease models and/or to assess the effectiveness of systems of gene therapy delivery. Gene expression of luminescent or fluorescent proteins can be detected directly on living animals by simply observing the associated optical signals by means of a cooled charged-coupled device camera. More accurate resolution can be obtained with more sophisticated technologies. Time-course and quasi-quantitative monitoring of the expression can be obtained on a given animal and followed on a large time window. The present paper describes the physical and technological methodologies and associated problems of in vivo optical imaging. Several examples of in vivo detection of gene delivery are described.

引用

收藏

页码：S85 / S91

页数：7

相关论文

共 30 条

[1] Biochemistry, mutagenesis, and oligomerization of DsRed, a red fluorescent protein from coral [J].

Baird, GS ;

Zacharias, DA ;

Tsien, RY .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (22) :11984-11989

[2] Optical imaging of Renilla luciferase reporter gene expression in living mice [J].

Bhaumik, S ;

Gambhir, SS .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (01) :377-382

[3] In vivo measurement of gene expression, angiogenesis and physiological function in tumors using multiphoton laser scanning microscopy [J].

Brown, EB ;

Campbell, RB ;

Tsuzuki, Y ;

Xu, L ;

Carmeliet, P ;

Fukumura, D ;

Jain, RK .

NATURE MEDICINE, 2001, 7 (07) :864-868

[4] Advances in vivo bioluminescence imaging of gene expression [J].

Contag, CH ;

Bachmann, MH .

ANNUAL REVIEW OF BIOMEDICAL ENGINEERING, 2002, 4 :235-260

[5] Noninvasive imaging of lentiviral-mediated reporter gene expression in living mice [J].

De, A ;

Lewis, XZ ;

Gambhir, SS .

MOLECULAR THERAPY, 2003, 7 (05) :681-691

[6] Quantitative imaging of cis-regulatory reporters in living embryos [J].

Dmochowski, IJ ;

Dmochowski, JE ;

Oliveri, P ;

Davidson, EH ;

Fraser, SE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (20) :12895-12900

[7] A submillimeter resolution fluorescence molecular imaging system for small animal imaging [J].

Graves, EE ;

Ripoll, J ;

Weissleder, R ;

Ntziachristos, V .

MEDICAL PHYSICS, 2003, 30 (05) :901-911

[8] GFP-like chromoproteins as a source of far-red fluorescent proteins [J].

Gurskaya, NG ;

Fradkov, AF ;

Terskikh, A ;

Matz, MV ;

Labas, YA ;

Martynov, VI ;

Yanushevich, YG ;

Lukyanov, KA ;

Lukyanov, SA .

FEBS LETTERS, 2001, 507 (01) :16-20

[9] Pictures in molecular medicine -: Watching real-time metastasis in vivo [J].

Hoffman, RM .

TRENDS IN MOLECULAR MEDICINE, 2002, 8 (07) :354-355

[10] Imaging transgene expression in live animals [J].

Honigman, A ;

Zeira, E ;

Ohana, P ;

Abramovitz, R ;

Tavor, E ;

Bar, I ;

Zilberman, Y ;

Rabinovsky, R ;

Gazit, D ;

Joseph, A ;

Panet, A ;

Shai, E ;

Palmon, A ;

Laster, M ;

Galun, E .

MOLECULAR THERAPY, 2001, 4 (03) :239-249