2-Methoxyestradiol inhibits superoxide anion generation while it enhances superoxide dismutase activity in swine granulosa cells

被引：14

作者：

Basini, Giuseppina ^{[1
]}

Santini, Sujen Eleonora ^{[1
]}

Grasselli, Francesca ^{[1
]}

机构：

[1] Univ Parma, Dipartimento Prod Anim Biotecnol Vet, Sez Fisiol, Fac Med Vet, I-43100 Parma, Italy

来源：

SIGNAL TRANSDUCTION PATHWAYS, PT B: STRESS SIGNALING AND TRANSCRIPTIONAL CONTROL | 2006年 / 1091卷

关键词：

2-methoxyestradiol; granulosa cells; angiogenesis; O-2(-); SOD;

D O I：

10.1196/annals.1378.052

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

2-Methoxyestradiol (2-ME) is an estradiol metabolite with antiangiogenic properties. It can be produced. by granulosa cells and it is present in normal follicle at high concentrations. The identification of reactive oxygen species (ROS) role in the molecular pharmacology of 2-ME is an active area Of research. The objective of this article was therefore to evaluate the effect of 2-ME on both superoxide anion (02) production and superoxide dismutase (SOD) activity in swine granulosa cells collected from follicles greater than 5 turn and treated for 48 h with 1 mu M 2-ME. 2-ME inhibited (P < 0-001) O-2- generation in swine granulosa cells, while it stimulated (P < 0.05) the SOD activity. We previously demonstrated that a stimulation of O-2 - generation triggers angiogenetic response in granulosa cells. Therefore, we argue that the inhibitory effect of 2-ME on O-2 - could be responsible for its antiangiogenetic effect.

引用

页码：34 / 40

页数：7

共 17 条

[1] Effect of reduced oxygen tension on reactive oxygen species production and activity of antioxidant enzymes in swine granulosa cells [J].