Same serial section correlative light and energy-filtered transmission electron microscopy

被引：21

作者：

Ren, Y ^{[1
]}

Kruhlak, MJ ^{[1
]}

Bazett-Jones, DP ^{[1
]}

机构：

[1] Hosp Sick Children, Cell Biol Programme, Toronto, ON M5G 1X8, Canada

来源：

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY | 2003年 / 51卷 / 05期

关键词：

fluorescence microscopy; energy-filtered transmission electron microscopy; electron spectroscopic imaging; nuclear structure; nuclear bodies; promyelocytic leukemia;

D O I：

10.1177/002215540305100506

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Correlative imaging of a specific cell with both the light microscope and the electron microscope has proved to be a difficult task, requiring enormous amounts of patience and technical skill. We describe a technique with a high rate of success, which can be used to identify a particular cell in the light microscope and then to embed and thin-section it for electron microscopy. The technique also includes a method to obtain many uninterrupted, thin serial sections for imaging by conventional or energy-filtered transmission electron microscopy, to obtain images for 3D analysis of detail at the suborganelle level.

引用

页码：605 / 612

页数：8

共 13 条

[1] Electron spectroscopic imaging of chromatin [J].

Bazett-Jones, DP ;

Hendzel, MJ .

METHODS-A COMPANION TO METHODS IN ENZYMOLOGY, 1999, 17 (02) :188-200

[2] Promyelocytic leukemia (PML) nuclear bodies are protein structures that do not accumulate RNA [J].

Boisvert, FM ;

Hendzel, MJ ;

Bazett-Jones, DP .

JOURNAL OF CELL BIOLOGY, 2000, 148 (02) :283-292

[3] GREEN FLUORESCENT PROTEIN AS A MARKER FOR GENE-EXPRESSION [J].

CHALFIE, M ;

TU, Y ;

EUSKIRCHEN, G ;

WARD, WW ;

PRASHER, DC .

SCIENCE, 1994, 263 (5148) :802-805

[4] Reduced mobility of the alternate splicing factor (ASF) through the nucleoplasm and steady state speckle compartments [J].

Kruhlak, MJ ;

Lever, MA ;

Fischle, W ;

Verdin, E ;

Bazett-Jones, DP ;

Hendzel, MJ .

JOURNAL OF CELL BIOLOGY, 2000, 150 (01) :41-51

[5] Molecular immunolabeling with recombinant single-chain variable fragment (scFv) antibodies designed with metal-binding domains [J].

Malecki, M ;

Hsu, A ;

Truong, L ;

Sanchez, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (01) :213-218

[6] Two-color GFP expression system for C-elegans [J].

Miller, DM ;

Desai, NS ;

Hardin, DC ;

Piston, DW ;

Patterson, GH ;

Fleenor, J ;

Xu, S ;

Fire, A .

BIOTECHNIQUES, 1999, 26 (05) :914-+

[7] POLEWARDS MICROTUBULE FLUX IN THE MITOTIC SPINDLE - EVIDENCE FROM PHOTOACTIVATION OF FLUORESCENCE [J].

MITCHISON, TJ .

JOURNAL OF CELL BIOLOGY, 1989, 109 (02) :637-652

[8] Bridging the resolution gap: Imaging the same transcription factories in cryosections by light and electron microscopy [J].

Pombo, A ;

Hollinshead, M ;

Cook, PR .

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1999, 47 (04) :471-480

[9]

Rieder CL, 1999, METHOD CELL BIOL, V61, P297

[10] In vivo localization of DNA sequences and visualization of large-scale chromatin organization using lac operator/repressor recognition [J].

Robinett, CC ;

Straight, A ;

Li, G ;

Willhelm, C ;

Sudlow, G ;

Murray, A ;

Belmont, AS .

JOURNAL OF CELL BIOLOGY, 1996, 135 (06) :1685-1700

← 1 2 →