Application and evaluation of ion-exchange UV spectrophotometric method for determination of sinapine in Brassica seeds and meals

被引：13

作者：

Wang, SX

Oomah, BD ^{[1
]}

McGregor, DI

机构：

[1] Agr & Agri Food Canada, Pacific Agrifood Res Ctr, Summerland, BC V0H 1Z0, Canada

[2] Agr & Agri Food Canada, Saskatoon Res Ctr, Saskatoon, SK S7N 0X2, Canada

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 1998年 / 46卷 / 02期

关键词：

sinapine determination; Brassica; rapeseed meat; cation-exchange column;

D O I：

10.1021/jf9705577

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

A cation-exchange column packed with CM-Sephadex C25 matrix was evaluated for its efficiency, capacity, elution volume, and reproducibility in purifying crude extracts of rapeseed or meal for the determination of sinapine content. Combined with a single extraction and ion-exchange column purification procedure, sinapine content in the seed or meal could be quantified with a coefficient of variance of 3.9%. Recovery of sinapine from rapeseed or meal using this method was 95-114% with a lower limit of the sinapine detection of 2.5 mu g/mL. The proposed method is not labor-intensive and is applicable to batch analysis of up to 120 samples per day, thereby reducing the cost of analysis.

引用

页码：575 / 579

页数：5

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