Cytotoxicity of zinc-containing bioactive glasses in contact with human osteoblasts

被引:130
作者
Aina, Valentina
Perardi, Alessandra
Bergandi, Loredana
Malavasi, Gianluca
Menabue, Ledi
Morterra, Claudio
Ghigo, Dario
机构
[1] Univ Turin, Dept Genet Biol & Biochem, I-10126 Turin, Italy
[2] Univ Turin, Dept Chem, IFM, I-10126 Turin, Italy
[3] Univ Turin, Ctr Excellence NIS, I-10126 Turin, Italy
[4] Univ Modena, Dept Chem, I-41100 Modena, Italy
[5] SCS Ctr, I-41100 Modena, Italy
关键词
bioactive glasses; zinc; cytotoxicity; oxidative stress; ion release; surface area;
D O I
10.1016/j.cbi.2007.03.002
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Bioactive glasses such as Hench's 45S5 have applications to tissue engineering and bone repair: the insertion of zinc has been proposed to improve their bone-bonding ability and to slacken their dissolution in extracellular body fluids. In view of a potential clinical application, we have investigated whether zinc-containing 45S5 (HZ) glasses might be cytotoxic for human MG-63 osteoblasts. In our experimental conditions, after 24 h of incubation HZ glasses released significant amounts of Zn2+ and induced in MG-63 cells release of lactate dehydrogenase (index of cytotoxicity) and the following indexes of oxidative stress: (i) accumulation of intracellular malonyldialdehyde, (ii) increased activity of pentose phosphate pathway, (iii) increased expression of heme oxygenase-1, (iv) increased activity of Cu,Zn-superoxide dismutase, (v) decreased level of intracellular thiols. These effects were inversely related to the zinc content of glass powders, were mimicked by ZnCl2 solutions and were prevented by either metal chelators (EDTA, NTA) or the antioxidant ascorbate, suggesting that Zn2+ released fastly from HZ glasses can cause MG-63 cell damage via an oxidative stress. This work highlights the importance of designing Zn-containing bioactive glasses without cytotoxic effects and gives supplementary information about the prooxidant role of zinc in living systems. (C) 2007 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:207 / 218
页数:12
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