Capillary electrophoretic separation of mono- and dinucleotides of adenosine using cyclodextrin solutions with MgCl2 additive

The capillary electrophoretic separation of adenine, adenosine and nucleotide isomers of adenosine has been studied using several kinds of buffer solutions with cyclodextrin and metal additives in the presence and absence of sodium dodecyl sulfate. Optimum resolution of 2',5'- and 3',5'-adenylyladenosine monophosphate was obtained using beta-cyclodextrin solution containing 10(-4) M MgCl2. The mixture of adenine, adenosine and nine types of adenosine nucleotide was able to be separated simultaneously within 15 min under the optimum conditions. The method was applicable to the analysis of the simulation reaction for the prebiotic formation of adenosine nucleotides. (C) 1998 Elsevier Science B.V.