NMR study on the hydroxy protons of the Lewis X and Lewis Y oligosaccharides

被引：15

作者：

Bekiroglu, S ^{[1
]}

Kenne, L ^{[1
]}

Sandström, C ^{[1
]}

机构：

[1] Swedish Univ Agr Sci, Dept Chem, SE-75007 Uppsala, Sweden

来源：

CARBOHYDRATE RESEARCH | 2004年 / 339卷 / 14期

关键词：

conformation; hydration; hydroxy protons; Lewis X; Lewis Y; NMR;

D O I：

10.1016/j.carres.2004.07.002

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The H-1 NMR chemical shifts and NOEs of hydroxy protons in Lewis X trisaccharide, beta-D-Galp-(1 --> 4)[alpha-L-Fucp-(1 -->3) ]-beta-D-GlcpNAc, and Lewis Y tetra sacch aride, alpha-L-Fucp-(1 --> 2)-beta-D-Galp-(1 --> 4)[alpha-L-Fucp-(1 --> 3)]-beta-D-GlcpNAc, were obtained for 85% H2O/15% (CD3)(2)CO solutions. The OH-4 signal of Galp in Lewis X and OH-3, OH-4 signals of Gall), and OH-2 signal of Fucp linked to Galp in Lewis Y had chemical shifts which indicate reduced hydration due to their proximity to the hydrophobic face of the Fucp unit linked to GlcpNAc. The inter-residue NOEs involving the exchangeable NH and OH protons confirmed the stacking interaction between the Fucp linked to the GlcpNAc and the Gall) residues in Lewis X and Lewis Y. (C) 2004 Elsevier Ltd. All rights reserved.

引用

页码：2465 / 2468

页数：4

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