Genomic representations using concatenates of Type IIB restriction endonuclease digestion fragments

被引：17

作者：

Tengs, T

LaFramboise, T

Den, RB

Hayes, DN

Zhang, JH

DebRoy, S

Gentleman, RC

O'Neill, K

Birren, B

Meyerson, M ^{[1
]}

机构：

[1] Dana Farber Canc Inst, Dept Med Oncol, Boston, MA 02115 USA

[2] Harvard Univ, Sch Med, Dept Pathol, Boston, MA 02115 USA

[3] Harvard Univ, Sch Publ Hlth, Dept Biostat, Boston, MA 02115 USA

[4] Harvard Univ, Broad Inst, Cambridge, MA 02141 USA

[5] MIT, Broad Inst, Cambridge, MA 02141 USA

来源：

NUCLEIC ACIDS RESEARCH | 2004年 / 32卷 / 15期

关键词：

D O I：

10.1093/nar/gnh120

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed a method for genomic representation using Type IIB restriction endonucleases. Representation by concatenation of restriction digests, or RECORD, is an approach to sample the fragments generated by cleavage with these enzymes. Here, we show that the RECORD libraries may be used for digital karyotyping and for pathogen identification by computational subtraction.

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页数：9

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