Novel insertion sequence-like elements in phytoplasma strains of the aster yellows group are putative new members of the IS3 family

被引:16
作者
Lee, IM [1 ]
Zhao, Y [1 ]
Bottner, KD [1 ]
机构
[1] USDA ARS, Mol Plant Pathol Lab, Beltsville, MD 20705 USA
关键词
insertion sequence; aster yellows phytoplasma group; peanut witches'-broom phytoplasma group; Mexican periwinkle virescence phytoplasma; group;
D O I
10.1016/j.femsle.2004.11.036
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Novel insertion sequence (IS)-like elements were isolated and characterized from phytoplasma strains in the aster yellows (AY) group (16SrI). the IS-like elements were cloned from phytoplasma strains AY1 and NJAY or PCR-amplified from 15 additional strains representing nine subgroups in the AY group using primers based on sequences of the putative transposases (Tpases). All IS-like elements contained sequences encoding similar Tpases of 321 amino acids (320 for strain CPh). Substantial amino acid sequence variability suggested multiple species of Tpases or IS-like elements exist in the AY phytoplasma group. These Tpases have an identical DDE motif that is most similar to the DDE consensus of Tpases in the IS3 family. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码:353 / 360
页数:8
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