Nucleation of insulin crystals in a wide continuous supersaturation gradient

被引：11

作者：

Penkova, A ^{[1
]}

Dimitrov, I ^{[1
]}

Nanev, C ^{[1
]}

机构：

[1] Bulgarian Acad Sci, Inst Phys Chem, BU-1113 Sofia, Bulgaria

来源：

TRANSPORT PHENOMENA IN MICROGRAVITY | 2004年 / 1027卷

关键词：

protein crystal nucleation; direct measurement with insulin;

D O I：

10.1196/annals.1324.006

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Modifying the classical double pulse technique, by using a supersaturation gradient along an insulin solution contained in a glass capillary tube, we found conditions appropriate for the direct measurement of nucleation parameters. The nucleation time lag has been measured. Data for the number of crystal nuclei versus the nucleation time were obtained for this hormone. Insulin was chosen as a model protein because of the availability of solubility data in the literature. A comparison with the results for hen-egg-white lysozyme, HEWL was performed.

引用

页码：56 / 63

页数：8

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