Detection of the esp gene in high-level gentamicin resistant Enterococcus faecalis strains from pet animals in Japan

被引:14
作者
Harada, T [1 ]
Tsuji, N [1 ]
Otsuki, K [1 ]
Murase, T [1 ]
机构
[1] Tottori Univ, Fac Agr, Lab Vet Microbiol, Tottori 6808553, Japan
关键词
Enterococcus; esp gene; pet animals; gentamicin; HLGR;
D O I
10.1016/j.vetmic.2004.12.012
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We investigated the prevalence of the esp gene and the susceptibility to gentamicin in Enterococcus faecalis and E. faecium strains obtained from pet animals. Nine of 30 E. faecalis and 2 of 38 E. faecium strains from the pet animals had the esp gene. Three esp-positive E. faecalis strains, which were isolated from two dogs and a cat, showed gentamicin MICs of >= 256 mu g/ml and harbored the high-level gentamicin resistance (HLGR) gene, aac(6')-Ie-aph(2")-Ia. Of the nine esp-positive E. faecalis strains, five, including the three strains with the HLGR gene, were closely related by numerical analysis of PFGE patterns. Longitudinal investigation needs to elucidate whether the HLGR gene was incorporated into a subpopulation of the esp-positive E. faecalis. (c) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码:139 / 143
页数:5
相关论文
共 22 条
[1]   Variant esp gene in vancomycin-sensitive Enterococcus faecium [J].
Baldassarri, L ;
Bertuccini, L ;
Ammendolia, MG ;
Gherardi, G ;
Creti, R .
LANCET, 2001, 357 (9270) :1802-1802
[2]   Automated ribotyping of vancomycin-resistant Enterococcus faecium isolates [J].
Brisse, S ;
Fussing, V ;
Ridwan, B ;
Verhoef, J ;
Willems, RJL .
JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (06) :1977-1984
[3]   Differences in antibiotic resistance patterns of Enterococcus faecalis and Enterococcus faecium strains isolated from farm and pet animals [J].
Butaye, P ;
Devriese, LA ;
Haesebrouck, F .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2001, 45 (05) :1374-1378
[4]   Aminoglycoside resistance in enterococci [J].
Chow, JW .
CLINICAL INFECTIOUS DISEASES, 2000, 31 (02) :586-589
[5]   A novel gentamicin resistance gene in Enterococcus [J].
Chow, JW ;
Zervos, MJ ;
Lerner, SA ;
Thal, LA ;
Donabedian, SM ;
Jaworski, DD ;
Tsai, S ;
Shaw, KJ ;
Clewell, DB .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1997, 41 (03) :511-514
[6]   DETECTION OF GLYCOPEPTIDE RESISTANCE GENOTYPES AND IDENTIFICATION TO THE SPECIES LEVEL OF CLINICALLY RELEVANT ENTEROCOCCI BY PCR [J].
DUTKAMALEN, S ;
EVERS, S ;
COURVALIN, P .
JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (01) :24-27
[7]   IDENTIFICATION OF ENTEROCOCCUS SPECIES ISOLATED FROM HUMAN INFECTIONS BY A CONVENTIONAL TEST SCHEME [J].
FACKLAM, RR ;
COLLINS, MD .
JOURNAL OF CLINICAL MICROBIOLOGY, 1989, 27 (04) :731-734
[8]   NUCLEOTIDE-SEQUENCE ANALYSIS OF THE GENE SPECIFYING THE BIFUNCTIONAL 6'-AMINOGLYCOSIDE ACETYLTRANSFERASE 2''-AMINOGLYCOSIDE PHOSPHOTRANSFERASE ENZYME IN STREPTOCOCCUS-FAECALIS AND IDENTIFICATION AND CLONING OF GENE REGIONS SPECIFYING THE 2 ACTIVITIES [J].
FERRETTI, JJ ;
GILMORE, KS ;
COURVALIN, P .
JOURNAL OF BACTERIOLOGY, 1986, 167 (02) :631-638
[9]   Prevalence of esp, encoding the enterococcal surface protein, in Enterococcus faecalis and Enterococcus faecium isolates from hospital patients, poultry, and pigs in Denmark [J].
Hammerum, AM ;
Jensen, LB .
JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (11) :4396-4396
[10]   Detection of the high-level aminoglycoside resistance gene aph(2")-Ib in Enterococcus faecium [J].
Kao, SJ ;
You, L ;
Clewell, DB ;
Donabedian, SM ;
Zervos, MJ ;
Petrin, J ;
Shaw, KJ ;
Chow, JW .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2000, 44 (10) :2876-2879