Evaluation of several methods to quantify immobilized proteins on gold and silica surfaces

被引：4

作者：

Orschel, M ^{[1
]}

Katerkamp, A ^{[1
]}

Meusel, M ^{[1
]}

Cammann, K ^{[1
]}

机构：

[1] Inst Chemosensorik & Biosensorik, D-48149 Munster, Germany

来源：

COLLOIDS AND SURFACES B-BIOINTERFACES | 1998年 / 10卷 / 05期

关键词：

biotin; gold surface; immobilization proteins; protein quantification; silica surface; streptavidin;

D O I：

10.1016/S0927-7765(98)00008-3

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

In the present study, the application of several methods for the determination of an immobilized protein are presented and the results discussed. Streptavidin was bound covalently on gold and silica surfaces. A radio assay was used as a reference method for the quantification of the immobilized protein. Two photometric, two fluorescence-spectroscopic methods and an immunochemical approach, based on a sandwich-ELISA (enzyme linked immunosorbent assay) format were applied with regard to their feasibility for sensitive protein quantification. The photometric methods were not sensitive enough, but one of the fluorescence based methods and the ELISA could be applied for the detection of low protein concentrations on the surfaces. (C) 1998 Elsevier Science B.V.

引用

页码：273 / 279

页数：7

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