Ethanol Extract of Fructus Ligustri Lucidi Promotes Osteogenesis of Mesenchymal Stem Cells

被引：67

作者：

Li, Guo ^{[1
]}

Zhang, Xiao-ai ^{[1
]}

Zhang, Jin-fang ^{[1
]}

Chan, Chu-yan ^{[1
,2
]}

Yew, David Tai Wai ^{[2
]}

He, Ming-liang ^{[1
]}

Lin, Marie Chia-mi ^{[3
]}

Leung, Ping-chung ^{[4
]}

Kung, Hsiang-fu ^{[4
]}

机构：

[1] Chinese Univ Hong Kong, Stanley Ho Ctr Emerging Infect Dis, Shatin, Hong Kong, Peoples R China

[2] Chinese Univ Hong Kong, Dept Anat, Shatin, Hong Kong, Peoples R China

[3] Univ Hong Kong, Dept Chem, Hong Kong, Hong Kong, Peoples R China

[4] Chinese Univ Hong Kong, Dept Orthopaed & Traumatol, Shatin, Hong Kong, Peoples R China

来源：

PHYTOTHERAPY RESEARCH | 2010年 / 24卷 / 04期

关键词：

bone marrow; Fructus Ligustri Lucidi; mesenchymal stem cells; osteogenesis; DIFFERENTIATION; PROLIFERATION; IMPROVEMENT; BALANCE; ACID; TBX3; RATS;

D O I：

10.1002/ptr.2987

中图分类号：

R914 [药物化学];

学科分类号：

100705 [微生物与生化药学];

摘要：

Fructus Ligustri Lucidi (FLL) has been used in traditional Chinese medicine for over 1000 years. The ethanol extract of FLL (EFLL) has been shown to be a potential candidate in the prevention and treatment of osteoporosis. The present study aimed to determine whether EFLL carries out the effect by promoting osteogenesis in mesenchymal stem cells (MSCs). The osteogenic differentiation of MSCs was evaluated by their alkaline phosphatase (ALP) activities and mineralization. Expression of genes was detected by RT-PCR. We found that EFLL significantly stimulated the ALP activities and shortened the time needed for the mineralization of MSCs during osteogenic differentiation. The expression of several osteoblast differentiation regulators was also upregulated by EFLL during this process. Our study demonstrated that the EFLL is capable of enhancing osteogenic differentiation of MSCs. It might be useful for treating diseases with inadequate bone formation, including osteoporosis. Copyright (C) 2009 John Wiley & Sons, Ltd.

引用

页码：571 / 576

页数：6

共 21 条

[1]

The potential of gene therapy for fracture healing in osteoporosis [J].

Egermann, M. ;

Schneider, E. ;

Evans, C. H. ;

Baltzer, A. W. .

OSTEOPOROSIS INTERNATIONAL, 2005, 16 (Suppl 2) :S120-S128

[2]

Canonical WNT signaling promotes osteogenesis by directly stimulating Runx2 gene expression [J].

Gaur, T ;

Lengner, CJ ;

Hovhannisyan, H ;

Bhat, RA ;

Bodine, PVN ;

Komm, BS ;

Javed, A ;

van Wijnen, AJ ;

Stein, JL ;

Stein, GS ;

Lian, JB .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (39) :33132-33140

[3]

Canonical Wnt signaling in differentiated osteoblasts controls osteoclast differentiation [J].

Glass, DA ;

Bialek, P ;

Ahn, JD ;

Starbuck, M ;

Patel, MS ;

Clevers, H ;

Taketo, MM ;

Long, FX ;

McMahon, AP ;

Lang, RA ;

Karsenty, G .

DEVELOPMENTAL CELL, 2005, 8 (05) :751-764

[4]

Signaling and transcriptional regulation in osteoblast commitment and differentiation [J].

Huang, Wei ;

Yang, Shuying ;

Shao, Jianzhong ;

Li, Yi-Ping .

FRONTIERS IN BIOSCIENCE-LANDMARK, 2007, 12 :3068-3092

[5]

Jaiswal N, 1997, J CELL BIOCHEM, V64, P295, DOI 10.1002/(SICI)1097-4644(199702)64:2<295::AID-JCB12>3.3.CO

[6]

2-6

[7]

Tbx3, a transcriptional factor, involves in proliferation and osteogenic differentiation of human adipose stromal cells [J].

Lee, Hee Sook ;

Cho, Hyun Hwa ;

Kim, Hoe Kyu ;

Bae, Yong Chan ;

Baik, Hyung Suk ;

Jung, Jin Sup .

MOLECULAR AND CELLULAR BIOCHEMISTRY, 2007, 296 (1-2) :129-136

[8]

Li M., 1994, CHINA J CHINESE MAT, V19, P504

[9]

Liang S.M., 1991, TONIC METHODS

[10]

MECC determination of oleanolic acid and ursolic acid isomers in Ligustrum lucidum Ait [J].

Liu, HX ;

Shi, YH ;

Wang, DX ;

Yang, GL ;

Yu, AM ;

Zhang, HQ .

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2003, 32 (03) :479-485

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