A direct comparison between fatty acid analysis and intact phospholipid profiling for microbial identification

被引：42

作者：

Fang, JS ^{[1
]}

Barcelona, MJ

Alvarez, PJJ

机构：

[1] Univ Michigan, Dept Civil & Environm Engn, Ann Arbor, MI 48109 USA

[2] Univ Iowa, Dept Civil & Environm Engn, Iowa City, IA 52242 USA

来源：

ORGANIC GEOCHEMISTRY | 2000年 / 31卷 / 09期

基金：

美国国家科学基金会;

关键词：

fatty acids; intact phospholipid profile; Pseudomonas; Burkholderia; GC/MS; LC/ESI/MS;

D O I：

10.1016/S0146-6380(00)00053-X

中图分类号：

P3 [地球物理学]; P59 [地球化学];

学科分类号：

0708 ; 070902 ;

摘要：

Two chemical methods for characterization of micro organisms were compared: phospholipid ester-linked fatty acid (PLFA) analysis by gas chromatography/mass spectrometry, and intact phospholipid profiling (IPP) using liquid chromatography/electrospray ionization/mass spectrometry. Both methods were tested on five reference pseudomonad strains: Pseudomonas putida mt-2, Pseudomonas putida F1, Burkholderia cepacia G4, Burkholderia pickettii PKO1, and Pseudomonas mendocina KR1. PLFA detected eight major fatty acids in these pseudomonads, ranging in chain length from C-14 to C-19. IPP detected 16 phospholipids in three different classes: phosphatidylglycerol, phosphatidylethanolamine and phosphatidyl-dimethylethanolamine. Factor analysis of the data showed that IPP is superior to the PLFA technique in microbial differentiation and identification. (C) 2000 Elsevier Science Ltd. All rights reserved.

引用

页码：881 / 887

页数：7

共 20 条

[1]

[Anonymous], 1979, METHODOLOGY BIOMASS

[2] EQUIVALENCE OF MICROBIAL BIOMASS MEASURES BASED ON MEMBRANE LIPID AND CELL-WALL COMPONENTS, ADENOSINE-TRIPHOSPHATE, AND DIRECT COUNTS IN SUBSURFACE AQUIFER SEDIMENTS [J].

BALKWILL, DL ;

LEACH, FR ;

WILSON, JT ;

MCNABB, JF ;

WHITE, DC .

MICROBIAL ECOLOGY, 1988, 16 (01) :73-84

[3] DOUBLE-BOND LOCATION IN MONOUNSATURATED FATTY-ACIDS BY DIMETHYL DISULFIDE DERIVATIZATION AND MASS-SPECTROMETRY - APPLICATION TO ANALYSIS OF FATTY-ACIDS IN PHEROMONE GLANDS OF 4 LEPIDOPTERA [J].

DUNKELBLUM, E ;

TAN, SH ;

SILK, PJ .

JOURNAL OF CHEMICAL ECOLOGY, 1985, 11 (03) :265-277

[4] Structural determination and quantitative analysis of bacterial phospholipids using liquid chromatography electrospray ionization mass spectrometry [J].

Fang, JS ;

Barcelona, MJ .

JOURNAL OF MICROBIOLOGICAL METHODS, 1998, 33 (01) :23-35

[5] The use of a classic lipid extraction method for simultaneous recovery of organic pollutants and microbial lipids from sediments [J].

Fang, JS ;

Findlay, RH .

JOURNAL OF MICROBIOLOGICAL METHODS, 1996, 27 (01) :63-71

[6]

Findlay R., 1996, MOL MICROBIAL ECOLOG, P1

[7] RESPONSE OF A BENTHIC MICROBIAL COMMUNITY TO BIOTIC DISTURBANCE [J].

FINDLAY, RH ;

TREXLER, MB ;

WHITE, DC .

MARINE ECOLOGY PROGRESS SERIES, 1990, 62 (1-2) :135-148

[8] ACCURACY, REPRODUCIBILITY, AND INTERPRETATION OF FATTY-ACID METHYL-ESTER PROFILES OF MODEL BACTERIAL COMMUNITIES [J].

HAACK, SK ;

GARCHOW, H ;

ODELSON, DA ;

FORNEY, LJ ;

KLUG, MJ .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1994, 60 (07) :2483-2493

[9] ADAPTATION OF PSEUDOMONAS-PUTIDA S12 TO ETHANOL AND TOLUENE AT THE LEVEL OF FATTY-ACID COMPOSITION OF MEMBRANES [J].

HEIPIEPER, HJ ;

DEBONT, JAM .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1994, 60 (12) :4440-4444

[10] THE APPLICATION OF ELECTRONIC-COMPUTERS TO FACTOR-ANALYSIS [J].

KAISER, HF .

EDUCATIONAL AND PSYCHOLOGICAL MEASUREMENT, 1960, 20 (01) :141-151

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