The Euplotes La motif protein p43 has properties of a telomerase-specific subunit

被引:47
作者
Aigner, S
Postberg, J
Lipps, HJ
Cech, TR [1 ]
机构
[1] Univ Colorado, Dept Chem & Biochem, Boulder, CO 80309 USA
[2] Univ Colorado, Howard Hughes Med Inst, Boulder, CO 80309 USA
[3] Univ Witten Herdecke, Inst Cell Biol, D-58453 Witten, Germany
关键词
D O I
10.1021/bi034121y
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Telomerase is a specialized reverse transcriptase synthesizing DNA repeats at telomeres. In addition to the RNA and catalytic protein components, telomerase from the ciliate Euplotes aediculatus contains the subunit p43. This protein is homologous to the La autoantigen, functioning in maturation of RNA polymerase III transcripts. Here we provide evidence that p43 is primarily associated with the telomerase ribonucleoprotein in vivo. Recombinant p43 binds telomerase RNA with low-nanomolar affinity in vitro, recognizing stem I and adjacent nucleotides or structures in the core of the RNA. Unlike authentic La proteins, p43 does not bind strongly to RNA polymerase III precursor transcripts and does not exhibit a marked binding preference for 3'-terminal oligouridylate residues. In isolated macronuclei, p43 largely colocalizes with telomerase RNA in discrete foci. These findings suggest that p43 is not the Euplotes La protein but instead plays a dedicated role in telomerase assembly and/or function. Thus, p43 joins the telomerase reverse transcriptase and the yeast proteins Est1p and Est3p as the only telomerase-specific proteins identified so far.
引用
收藏
页码:5736 / 5747
页数:12
相关论文
共 71 条
[1]   Euplotes telomerase contains an La motif protein produced by apparent translational frameshifting [J].
Aigner, S ;
Lingner, J ;
Goodrich, KJ ;
Grosshans, CA ;
Shevchenko, A ;
Mann, M ;
Cech, TR .
EMBO JOURNAL, 2000, 19 (22) :6230-6239
[2]   DEVELOPMENT OF MACRONUCLEUS IN CILIATED PROTOZOAN STYLONYCHIA-MYTILUS [J].
AMMERMANN, D ;
STEINBRUCK, G ;
BERGER, LV ;
HENNIG, W .
CHROMOSOMA, 1974, 45 (04) :401-429
[3]   Analysis of the structure of human telomerase RNA in vivo [J].
Antal, M ;
Boros, É ;
Solymosy, F ;
Kiss, T .
NUCLEIC ACIDS RESEARCH, 2002, 30 (04) :912-920
[4]   The product of the Survival of Motor Neuron (SMN) gene is a human telomerase-associated protein [J].
Bachand, F ;
Boisvert, FM ;
Côté, J ;
Richard, S ;
Autexier, C .
MOLECULAR BIOLOGY OF THE CELL, 2002, 13 (09) :3192-3202
[5]   Reconstitution of human telomerase activity in vitro [J].
Beattie, TL ;
Zhou, W ;
Robinson, MO ;
Harrington, L .
CURRENT BIOLOGY, 1998, 8 (03) :177-180
[6]   ARCHITECTURE OF TELOMERASE RNA [J].
BHATTACHARYYA, A ;
BLACKBURN, EH .
EMBO JOURNAL, 1994, 13 (23) :5721-5731
[7]  
BOELENS WC, 1995, RNA, V1, P273
[8]   DIRECT INTERACTIONS BETWEEN AUTOANTIGEN LA AND HUMAN-IMMUNODEFICIENCY-VIRUS LEADER RNA [J].
CHANG, YN ;
KENAN, DJ ;
KEENE, JD ;
GATIGNOL, A ;
JEANG, KT .
JOURNAL OF VIROLOGY, 1994, 68 (11) :7008-7020
[9]  
Chapon C, 1997, RNA, V3, P1337
[10]   Secondary structure of vertebrate telomerase RNA [J].
Chen, JL ;
Blasco, MA ;
Greider, CW .
CELL, 2000, 100 (05) :503-514