Optically encoded particles and their applications in multiplexed biomedical assays

In the future, the rapid discovery of new cures, vaccines, and diagnostics for common diseases will depend on the ability of biomedical researchers to investigate complex mixtures of proteins or DNA. The need to measure the abundance of these entities, together with their level of interaction, has driven the development of new research tools that enable simultaneous analysis of multiple analytes (multiplexing). Optically encoded particles are emerging as the multiplexing tools of choice, especially for clinical research. In this Review, an overview of various new optical encoding methods will be presented, together with important biomedical applications in which particle-based assays are currently being used.