Live-cell reporters for fluorescence imaging

被引：28

作者：

Correa, Ivan R., Jr. ^{[1
]}

机构：

[1] New England Biolabs Inc, Ipswich, MA 01938 USA

来源：

CURRENT OPINION IN CHEMICAL BIOLOGY | 2014年 / 20卷

关键词：

FUSION PROTEINS; TAG; REVEALS; PROBES; DIMERIZATION; SPECIFICITY; FLUOROPHORE; ACTIVATION; MICROSCOPY; STABILITY;

D O I：

10.1016/j.cbpa.2014.04.007

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Advances in the development of new fluorescent reporters and imaging techniques have revolutionized our ability to directly visualize biological processes in living systems. Real-time analysis of protein localization, dynamics, and interactions has been made possible by site-specific protein labeling with custom designed probes. This review outlines some of the most recent advances in the design and application of live-cell imaging probes, with a particular focus on SNAP-tag technology. Specific examples illustrating applications in superresolution and single-molecule imaging, protein trafficking and recycling, and protein-protein interactions are presented.

引用

收藏

页码：36 / 45

页数：10

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