Fusarium udum is resistant to the mycolytic activity of a biocontrol strain of Bacillus subtilis AF 1

被引:24
作者
Harish, S [1 ]
Manjula, K [1 ]
Podile, AR [1 ]
机构
[1] Univ Hyderabad, Dept Plant Sci, Hyderabad 500046, Andhra Pradesh, India
关键词
biological control; Fusarium udum; Bacillus subtilis; mycolysis;
D O I
10.1111/j.1574-6941.1998.tb00490.x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
In vitro interaction of the pigeonpea wilt pathogen Fusarium udum and a biocontrol strain of Bacillus subtilis AF 1 showed that the fungus forms chlamydospore-like structures and increases vacuolation, when both cultures are simultaneously inoculated into potato dextrose broth. If B. subtilis AF 1 was inoculated after 24 h of growth of F. udum, chlamydospores were not formed and regular conidiation was observed. The growth of F. udum was significantly affected in the presence of AF 1 in terms of dry weight. Extracellular proteins of B. subtilis AF 1 reduced the growth of F. udum in proportion to the concentration of the protein precipitate. Cell-free filtrates of B. subtilis AF 1 prepared from chitin-containing medium showed chitinase activity. The formation of chlamydospore-like structures and vacuolated portions in mycelium of F. udum in the presence of AF 1 suggests that F. udum has a mechanism to tolerate mycolytic activity and grows slowly with regular conidiation. (C) 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V.
引用
收藏
页码:385 / 390
页数:6
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