A NAC domain protein interacts with Tomato leaf cuil virus replication accessory protein and enhances viral replication

被引：137

作者：

Selth, LA

Dogra, SC

Rasheed, MS

Healy, H

Randles, JW

Rezaian, MA ^{[1
]}

机构：

[1] CSIRO, Hort Unit, Glen Osmond, SA 5064, Australia

[2] Univ Adelaide, Sch Agr & Wine, Adelaide, SA 5005, Australia

[3] Natl Inst Biotechnol & Genet Engn, Faisalabad, Pakistan

来源：

PLANT CELL | 2005年 / 17卷 / 01期

关键词：

D O I：

10.1105/tpc.104.027235

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Geminivirus replication enhancer (REn) proteins dramatically increase the accumulation of viral DNA species by an unknown mechanism. In this study, we present evidence implicating SINAC1, a new member of the NAC domain protein family from tomato (Solanum lycopersicum), in Tomato leaf curl virus (TLCV) REn function. We isolated SINAC1 using yeast (Saccharomyces cerevisiae) two-hybrid technology and TLCV REn as bait, and confirmed the interaction between these proteins in vitro. TLCV induces SINAC1 expression specifically in infected cells, and this upregulation requires REn. In a transient TLCV replication system, overexpression of SINAC1 resulted in a substantial increase in viral DNA accumulation. SINAC1 colocalized with REn to the nucleus and activated transcription of a reporter gene in yeast, suggesting that in healthy cells it functions as a transcription factor. Together, these results imply that SINAC1 plays an important role in the process by which REn enhances TLCV replication.

引用

收藏

页码：311 / 325

页数：15

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