Isolation, cultivation and characterization of adult murine prostate stem cells

被引：140

作者：

Lukacs, Rita U. ^{[1
]}

Goldstein, Andrew S. ^{[2
]}

Lawson, Devon A. ^{[1
]}

Cheng, Donghui ^{[3
]}

Witte, Owen N. ^{[1
,3
,4
,5
]}

机构：

[1] Univ Calif Los Angeles, Dept Microbiol Mol Genet & Immunol, Los Angeles, CA 90024 USA

[2] Univ Calif Los Angeles, Inst Mol Biol, Los Angeles, CA 90024 USA

[3] Univ Calif Los Angeles, Howard Hughes Med Inst, Los Angeles, CA 90024 USA

[4] Univ Calif Los Angeles, David Geffen Sch Med, Dept Med & Mol Pharmacol, Los Angeles, CA 90095 USA

[5] Univ Calif Los Angeles, Eli & Edythe Broad Ctr Regenerat Med & Stem Cell, Los Angeles, CA USA

来源：

NATURE PROTOCOLS | 2010年 / 5卷 / 04期

基金：

美国国家科学基金会;

关键词：

CANCER PROGRESSION; ANDROGEN-RECEPTOR; SUBPOPULATION; BIOLOGY; TISSUE; ORIGIN; MARKER;

D O I：

10.1038/nprot.2010.11

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

The successful isolation and cultivation of prostate stem cells will allow us to study their unique biological properties and their application in therapeutic approaches. Here we describe step-by-step procedures on the basis of previous work in our laboratory for the harvesting of primary prostate cells from adolescent male mice by a modified enzymatic procedure; the isolation of an enriched population of prostate stem cells through cell sorting; and the cultivation of prostate stem cells in vitro and characterization of these cells and their stem-like activity, including in vivo tubule regeneration. Normally, it will take approximately 8 h to harvest prostate cells, isolate the stem-cell-enriched population and set up the in vitro sphere assay. It will take up to 8 weeks to analyze the unique properties of the stem cells, including their regenerative capacity in vivo.

引用

页码：702 / 713

页数：12

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