Expression of Helios, an Ikaros Transcription Factor Family Member, Differentiates Thymic-Derived from Peripherally Induced Foxp3+ T Regulatory Cells

被引：1050

作者：

Thornton, Angela M. ^{[1
]}

Korty, Patricia E. ^{[1
]}

Tran, Dat Q. ^{[1
]}

Wohlfert, Elizabeth A. ^{[2
]}

Murray, Patrick E. ^{[1
]}

Belkaid, Yasmine ^{[2
]}

Shevach, Ethan M. ^{[1
]}

机构：

[1] NIAID, Immunol Lab, NIH, Bethesda, MD 20892 USA

[2] NIAID, Parasit Dis Lab, NIH, Bethesda, MD 20892 USA

来源：

JOURNAL OF IMMUNOLOGY | 2010年 / 184卷 / 07期

基金：

美国国家卫生研究院;

关键词：

TGF-BETA; IN-VIVO; GENE; ACTIVATION; GENERATION; INDUCTION; ABSENCE; SELF; LYMPHOCYTES; ENTEROPATHY;

D O I：

10.4049/jimmunol.0904028

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Helios, a member of the Ikaros transcription factor family, is preferentially expressed at the mRNA level by regulatory T cells (Treg cells). We evaluated Helios protein expression using a newly generated mAb and demonstrated that it is expressed in all thymocytes at the double negative 2 stage of thymic development. Although Helios was expressed by 100% of CD4(+)CD8(-)Foxp3(+) thymocytes, its expression in peripheral lymphoid tissues was restricted to a subpopulation (similar to 70%) of Foxp3(+) T cells in mice and humans. Neither mouse nor human naive T cells induced to express Foxp3 in vitro by TCR stimulation in the presence of TGF-beta expressed Helios. Ag-specific Foxp3(+) T cells induced in vivo by Ag feeding also failed to express Helios. Collectively, these results demonstrate that Helios is potentially a specific marker of thymic-derived Treg cells and raises the possibility that a significant percentage of Foxp3(+) Treg cells are generated extrathymically. The Journal of Immunology, 2010, 184: 3433-3441.

引用

页码：3433 / 3441

页数：9

共 46 条

[1] Activation-induced FOXP3 in human T effector cells does not suppress proliferation or cytokine production [J].