Increased gene expression and production of murine endothelin receptors after birth

被引：24

作者：

Adur, J

Takizawa, S

Quan, JX

Uchide, T

Saida, K ^{[1
]}

机构：

[1] Natl Inst Adv Ind Sci & Technol, Inst Biol Resources & Funct, Tsukuba, Ibaraki 3058566, Japan

[2] Natl Univ Entre Rios, Microscopy Lab, Sch Bioengn, RA-3101 Oro Verde, Entre Rios, Argentina

[3] Kitasato Univ, Dept Toxicol, Sch Vet Med & Anim Sci, Towada, Aomori 0348628, Japan

[4] Natl Inst Adv Ind Sci & Technol, Human Stress Signal Res Ctr, Ikeda, Osaka 5368577, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2003年 / 305卷 / 03期

基金：

日本学术振兴会;

关键词：

endothelin receptors; gene expression; mouse development; real-time PCR; 2(-delta delta C(T)) method; (poly)peptide production;

D O I：

10.1016/S0006-291X(03)00769-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We developed the real-time PCR quantification of endothelin-A (ET-A) and endothelin-13 (ET-B) receptor genes and present their relative expression levels in various adult tissues and during development in mouse using the 2(-Delta Delta C(T)) method. ET-A and ET-B receptors were detected in all tissues examined. Gene expression of ET-A and ET-B receptors increases during the later stages of embryonic development in lung, heart, liver, kidney, and skin and reaches a maximum on the first one or two days after birth. The results, in agreement with our data on endothelin (ET) ligands, suggest that the ET system may be involved in the emergence and maintenance of functions vital after birth in these organs. These findings were corroborated through observation of the correlation between the gene expression and (poly)peptide production of the ET system in normal skin before and after parturition. (C) 2003 Elsevier Science (USA). All rights reserved.

引用

页码：700 / 706

页数：7

共 33 条

[1] MUTATION OF THE ENDOTHELIN-RECEPTOR-B GENE IN WAARDENBURG-HIRSCHSPRUNG-DISEASE [J].