RGD-functionalized spherulites™ as targeted vectors captured by adherent cultured cells

被引：24

作者：

Chenevier, P

Delord, B

Amédée, J

Bareille, R

Ichas, F

Roux, D

机构：

[1] Ctr Rech Paul Pascal, F-33600 Pessac, France

[2] Univ Bordeaux 2, INSERM, U443, F-33076 Bordeaux, France

[3] Univ Bordeaux 1, IECB, F-33405 Talence, France

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH | 2002年 / 1593卷 / 01期

关键词：

targeting; controlled release; RGD;

D O I：

10.1016/S0167-4889(02)00313-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Spherulites are multilamellar vesicles consisting of concentric shells that can encapsulate small organic molecules or macromolecules. We investigate the possibility of targeting neutral spherulites to adherent culture cells by functionalizing their surface with RGD-containing ligands. The strength and specificity of association of RGD spherulites with several cell lines (EAhy 926 endothelial cell line, human umbilical vein endothelial cell (HUVEC) and human osteoprogenitor (HOP) primary cells) was studied, and the molecular interaction of RGD spherulites with the EAhy 926 cell surface was investigated. We show that, after binding to cells, spherulites are internalized. (C) 2002 Elsevier Science B.V. All fights reserved.

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页码：17 / 27

页数：11

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