A novel protein phosphatase 2C family member (PP2Cζ) is able to associate with ubiquitin conjugating enzyme 9

被引：16

作者：

Kashiwaba, M

Katsura, K

Ohnishi, M

Sasaki, M

Tanaka, H

Nishimune, Y

Kobayashi, T

Tamura, S

机构：

[1] Tohoku Univ, Dept Biochem, Inst Dev Aging & Canc, Aoba Ku, Sendai, Miyagi 9808575, Japan

[2] Hirosaki Univ, Sch Med, Dept Surg 2, Hirosaki, Aomori 0368562, Japan

[3] Japan Int Res Ctr Agr Sci, Biol Resources Div, Tsukuba, Ibaraki 3050851, Japan

[4] Chubu Univ, Coll Biosci & Biotechnol, Dept Biol Chem, Kasugau 4878501, Japan

[5] Osaka Univ, Dept Sci Lab Anim Expt, Res Inst Microbial Dis, Suita, Osaka 5650871, Japan

来源：

FEBS LETTERS | 2003年 / 538卷 / 1-3期

关键词：

protein phosphatase 2C; testicular germ cell; yeast two-hybrid screening; ubiquitin conjugating enzyme 9; small ubiquitin-related modifier-1;

D O I：

10.1016/S0014-5793(03)00153-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study we have cloned a novel member of mouse protein phosphatase 2C family, PP2Czeta, which is composed of 507 amino acids and has a unique N-terminal region. The overall similarity of the amino acid sequence between PP2Czeta and PP2Calpha was 22%. On Northern blot analysis PP2Czeta was found to be expressed specifically in the testicular germ cells. PP2Czeta expressed in COS7 cells was able to associate with ubiquitin conjugating enzyme 9 (UBC9) and the association was enhanced by co-expression of small ubiquitin-related modifier-1 (SUMO-1), suggesting that PP2Czeta exhibits its specific role through its SUMO-induced recruitment to UBC9. (C) 2003 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.

引用

页码：197 / 202

页数：6

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