Ethylene signalling and ethylene-targeted transcription factors are required to balance beneficial and nonbeneficial traits in the symbiosis between the endophytic fungus Piriformospora indica and Arabidopsis thaliana

被引:134
作者
Camehl, Iris [1 ]
Sherameti, Irena [1 ]
Venus, Yvonne [1 ]
Bethke, Gerit [2 ]
Varma, Ajit [3 ]
Lee, Justin [2 ]
Oelmueller, Ralf [1 ]
机构
[1] Univ Jena, Inst Allgemeine Botan & Pflanzenphysiol, D-07743 Jena, Germany
[2] Leibniz Inst Plant Biochem, Dept Stress & Dev Biol, D-06120 Halle, Germany
[3] Amity Univ, Amity Inst Microbial Sci, Noida 201303, UP, India
关键词
EIN2; EIN3; EIL1; ERF1; ethylene; ETR1; jasmonic acid; Piriformospora indica; plant-microbe interaction; RECEPTOR GENE FAMILY; F-BOX PROTEINS; RESPONSE PATHWAY; GCC-BOX; SYSTEMIC RESISTANCE; NICOTIANA-ATTENUATA; DISEASE RESISTANCE; BOTRYTIS-CINEREA; PLASMA-MEMBRANE; ROOT ENDOPHYTE;
D O I
10.1111/j.1469-8137.2009.03149.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
P>The endophytic fungus Piriformospora indica colonizes the roots of the model plant Arabidopsis thaliana and promotes its growth and seed production. The fungus can be cultivated in axenic culture without a host, and therefore this is an excellent system to investigate plant-fungus symbiosis. The growth of etr1, ein2 and ein3/eil1 mutant plants was not promoted or even inhibited by the fungus; the plants produced less seeds and the roots were more colonized compared with the wild-type. This correlates with a mild activation of defence responses. The overexpression of ETHYLENE RESPONSE FACTOR1 constitutively activated defence responses, strongly reduced root colonization and abolished the benefits for the plants. Piriformospora indica-mediated stimulation of growth and seed yield was not affected by jasmonic acid, and jasmonic acid-responsive promoter beta-glucuronidase gene constructs did not respond to the fungus in Arabidopsis roots. We propose that ethylene signalling components and ethylene-targeted transcription factors are required to balance beneficial and nonbeneficial traits in the symbiosis. The results show that the restriction of fungal growth by ethylene signalling components is required for the beneficial interaction between the two symbionts.
引用
收藏
页码:1062 / 1073
页数:12
相关论文
共 80 条
[1]   EIN2, a bifunctional transducer of ethylene and stress responses in Arabidopsis [J].
Alonso, JM ;
Hirayama, T ;
Roman, G ;
Nourizadeh, S ;
Ecker, JR .
SCIENCE, 1999, 284 (5423) :2148-2152
[2]   Piriformospora indica and Sebacina vermifera increase growth performance at the expense of herbivore resistance in Nicotiana attenuata [J].
Barazani, O ;
Benderoth, M ;
Groten, K ;
Kuhlemeier, C ;
Baldwin, IT .
OECOLOGIA, 2005, 146 (02) :234-243
[3]   Sebacina vermifera promotes the growth and fitness of Nicotiana attenuata by inhibiting ethylene signaling [J].
Barazani, Oz ;
von Dahl, Caroline C. ;
Baldwin, Ian T. .
PLANT PHYSIOLOGY, 2007, 144 (02) :1223-1232
[4]  
Becker C, 2006, CYTOTHERAPY, V8, P10
[5]  
Berrocal-Lobo M, 2004, MOL PLANT MICROBE IN, V17, P763, DOI 10.1094/MPMI.2004.17.7.763
[6]   Constitutive expression of ETHYLENE-RESPONSE-FACTOR1 in Arabidopsis confers resistance to several necrotrophic fungi [J].
Berrocal-Lobo, M ;
Molina, A ;
Solano, R .
PLANT JOURNAL, 2002, 29 (01) :23-32
[7]   Ethylene: A gaseous signal molecule in plants [J].
Bleecker, AB ;
Kende, H .
ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, 2000, 16 :1-+
[8]   INSENSITIVITY TO ETHYLENE CONFERRED BY A DOMINANT MUTATION IN ARABIDOPSIS-THALIANA [J].
BLEECKER, AB ;
ESTELLE, MA ;
SOMERVILLE, C ;
KENDE, H .
SCIENCE, 1988, 241 (4869) :1086-1089
[9]   The role of ethylene in host-pathoven interactions [J].
Broekaert, Willem F. ;
Delaure, Stijn L. ;
De Bolle, Miguel F. C. ;
Cammue, Bruno P. A. .
ANNUAL REVIEW OF PHYTOPATHOLOGY, 2006, 44 :393-416
[10]   INTERACTIONS OF ETHYLENE WITH CITRUS STEM-END ROT CAUSED BY DIPLODIA-NATALENSIS [J].
BROWN, GE ;
LEE, HS .
PHYTOPATHOLOGY, 1993, 83 (11) :1204-1208