alpha(5) subunit forms functional alpha(3)beta(4)alpha(5) nAChRs in transfected human cells

被引：41

作者：

Fucile, S

Barabino, B

Palma, E

Grassi, F

Limatola, C

Mileo, AM

Alema, S

Ballivet, M

Eusebi, F

机构：

[1] UNIV ROME,IST PASTEUR FONDAZ CENCI BOLOGNETTI,DIPARTIMENTO MED SPERIMENTALE & PATOL,ROME,ITALY

[2] IST REGINA ELENA,CTR RIC SPERIMENTALE,LAB BIOFIS,I-00158 ROME,ITALY

[3] CNR,IST BIOL CELLULARE,I-00137 ROME,ITALY

[4] UNIV GENEVA,DEPT BIOCHIM,CH-1211 GENEVA 4,SWITZERLAND

来源：

NEUROREPORT | 1997年 / 8卷 / 11期

关键词：

alpha(5) subunit; cell transfection; nicotinic acetylcholine receptor; oocytes; patch clamp;

D O I：

10.1097/00001756-199707280-00005

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

NACHRS heterologously expressed in human cells after transient transfection with alpha(3) beta(4) alpha(5) or alpha(3) beta(4) subunit cDNAs exhibited similar sensitivities to antagonists and comparable functional channel profiles. However, the sum of two Hill equations was required for best fitting the ACh dose-current response curves after co-expression of alpha(5), alpha(3) and beta(4) subunits. One component was comparable to that obtained in alpha(3) beta(4)-transfected cells, while the additional component, putatively attributed to an alpha(3) beta(4) alpha(5) nAChR population, showed a Hill coefficient >2 and a nine-fold greater half-maximal ACh concentration (EC50). These results suggest that the alpha(5) subunit participates in the assembly of alpha(3) beta(4) alpha(5) nAChRs complexes in human cells, adding a new member to the family of neuronal nicotinic receptors.

引用

页码：2433 / 2436

页数：4

共 17 条

[1] NEURONS CAN MAINTAIN MULTIPLE CLASSES OF NICOTINIC ACETYLCHOLINE-RECEPTORS DISTINGUISHED BY DIFFERENT SUBUNIT COMPOSITIONS [J].