Monitoring of antigen-specific CD8 T cells in patients with type 1 diabetes treated with antiCD3 monoclonal antibodies

被引：34

作者：

Cernea, Simonal ^{[1
]}

Herold, Kevan C. ^{[1
]}

机构：

[1] Yale Univ, Dept Immunobiol, New Haven, CT 06520 USA

来源：

CLINICAL IMMUNOLOGY | 2010年 / 134卷 / 02期

关键词：

Type; 1; diabetes; CD8 T cells; Tetramer; AntiCD3 monoclonal antibody; CLASS-II TETRAMERS; ANTI-CD3; MAB; EFFECTOR FUNCTIONS; VIRUS-INFECTION; SINGLE COURSE; RESPONSES; ONSET; INSULIN; MEMORY; MICE;

D O I：

10.1016/j.clim.2009.09.005

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The way in which anti-CD3 monoclonal antibodies (mAbs) modify human immune responses in type 1 diabetes (T1DM) is not known. We prepared a panel of Class 1 HLA-A2.1 tetramers with peptides from diabetes-associated antigens and studied the frequency and phenotype of the cells in patients with T1DM and blood donors and in patients treated with anti-CD3 mAb (Teplizumab). More patients with T1DM showed positive staining for at least 1 tetramer using frozen and fresh samples (p < 0.05). Three months following treatment with anti-CD3 mAb, the proportion of GAD65- and InsB-peptide reactive CD8+ T cells increased (p < 0.05). The phenotype of these cells was modulated from naive to effector memoryRA+. We concludethat Class 1 MHC tetramers can identify antigen specific CD8+ T cells in patients with T1DM. The frequency of certain specificities increases after treatment with anti-CD3 mAb. Their modulated phenotype may have functional consequences for their pathogenicity. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：121 / 129

页数：9

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