Lupus anticoagulant testing using plasma spiked with monoclonal antibodies: performance in the UKNEQAS proficiency testing programme

被引:27
作者
Jennings, I
Mackie, I
Arnout, J
Preston, FE
机构
[1] UK NEQAS Blood Coagulat, Sheffield S10 2QN, S Yorkshire, England
[2] UCL, Dept Haematol, London, England
[3] Katholieke Univ Leuven, Ctr Mol & Vasc Biol, Louvain, Belgium
关键词
lupus anticoagulant; monoclonal antibodies; quality control;
D O I
10.1111/j.1538-7836.2004.01028.x
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
We report here results from a United Kingdom National Quality Assessment Scheme (UK NEQAS) exercise in which both plasma spiked with monoclonal antibodies and plasma from a patient known to have lupus anticoagulant (LA) were distributed to 245 hemostasis laboratories with a request for them to test for possible LA using their routine screening procedure. In general, good agreement was seen in the diagnosis of samples spiked with monoclonal antibodies against beta2-glycoprotein 1 (beta2GP1) and prothrombin, the LA-positive patient sample, and a normal pooled plasma; over 87% of centers correctly identified each sample. However, methods employing platelet neutralizing procedures were associated with a higher proportion of false-negative responses with the antiprothrombin-spiked sample, and it is important to recognize that sensitivity and responsiveness of different methods may vary between artificial plasmas and different LA-positive patient plasmas.
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页码:2178 / 2184
页数:7
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