Isolation and characterisation of an aryl-β-D-glucoside uptake and utilisation system (abg) from the gram-positive ruminal Clostridium species C-longisporum

被引:27
作者
Brown, GD [1 ]
Thomson, JA [1 ]
机构
[1] Univ Cape Town, Dept Microbiol, ZA-7700 Rondebosch, South Africa
来源
MOLECULAR AND GENERAL GENETICS | 1998年 / 257卷 / 02期
关键词
Clostridium; aryl-beta-glucoside; PTS;
D O I
10.1007/s004380050641
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A phosphotransferase-dependent aryl-beta-glucoside uptake and utilisation system (abg) was isolated from the ruminal Clostridium ("C. longisporum"). The system is composed of three genes, abgG, abgF and abgA, and a number of regulatory regions, including terminator/antiterminator type stem-loop structures preceding the abgG and abgF genes. Similarity analysis of the proteins encoded by these genes indicated that they were responsible for the regulation of the abg system through antitermination (AbgG), the uptake and phosphorylation of aryl-beta-glucosides (AbgF) and the hydrolysis of the intracellular phosphorylated glycosides (AbgA). Experimental evidence for the functions of AbgF and AbgA was obtained. Although it was not possible to demonstrate any function for AbgG, a promoter 5' to the abgG gene was identified which was responsible for expression of the downstream genes. The abg system is remarkably similar to operons from the gram negative Enterobacteriaceae, both in the coding and non-coding regulatory regions.
引用
收藏
页码:213 / 218
页数:6
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