DNA-carrying latex particles for DNA diagnosis. 1. Separation of normal and point mutant DNAs according to the difference in hybridization efficiency

被引：13

作者：

Hatakeyama, M

Iwato, S

Fujimoto, K

Handa, H

Kawaguchi, H

机构：

[1] Keio Univ, Fac Sci & Technol, Dept Appl Chem, Kohoku Ku, Yokohama, Kanagawa 223, Japan

[2] Tokyo Inst Technol, Fac Biosci & Biotechnol, Dept Biomol Engn, Midori Ku, Yokohama, Kanagawa 226, Japan

来源：

COLLOIDS AND SURFACES B-BIOINTERFACES | 1998年 / 10卷 / 03期

关键词：

affinity latex; DNA diagnosis; hybridization; microsphere; point mutation;

D O I：

10.1016/S0927-7765(97)00059-3

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Point mutation of specific genome DNA causes cancer. A system for rapid and sensitive detection of specific genome DNA is required because it enables early detection and treatment of cancer. We developed a new system for DNA diagnosis using DNA-carrying latex particles and succeeded to collect point mutant specifically from the mixture with normal DNA of codon 12 of K-ras gene. (C) 1998 Elsevier Science B.V.

引用

收藏

页码：161 / 169

页数：9

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