Isolation and quantitation of phosphatidylcholine by reversed-phase liquid-liquid extraction

被引：14

作者：

Hradec, J ^{[1
]}

Dufek, P ^{[1
]}

机构：

[1] Inst Hematol & Blood Transfus, Dept Cellular Biochem, CR-12820 Prague 2, Czech Republic

来源：

JOURNAL OF CHROMATOGRAPHY B | 1997年 / 703卷 / 1-2期

关键词：

phosphatidylcholines;

D O I：

10.1016/S0378-4347(97)00401-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple and rapid method is described for the determination of total phosphatidylcholines in serum. Extracts of lipids are applied to octadecyl silica cartridges and phosphatidylcholines are eluted by methanol-acetonitrile mixtures containing choline. Quantitation of these compounds is performed by colorimetry of their complexes with erythrosin B. The method is sensitive down to approximately 10 mu g, exhibits good reproducibility and may be used as a preliminary step for the separation of individual molecular species of phosphatidylcholines by high-performance liquid chromatography. (C) 1997 Elsevier Science B.V.

引用

页码：259 / 262

页数：4

共 11 条

[1] REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF PHOSPHOLIPIDS WITH FLUORESCENCE DETECTION [J].

ABIDI, SL ;

MOUNTS, TL ;

RENNICK, KA .

JOURNAL OF CHROMATOGRAPHY, 1993, 639 (02) :175-184

[2] COLORIMETRIC PHOSPHOLIPID DETERMINATION WITH ERYTHROSIN-B [J].

ANDREE, HAM ;

SOEDJAK, HS .

ANALYTICAL BIOCHEMISTRY, 1994, 222 (02) :465-471

[3] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ANALYSIS OF PHOSPHOLIPIDS FROM DIFFERENT SOURCES WITH COMBINED FLUORESCENCE AND ULTRAVIOLET DETECTION [J].

BERNHARD, W ;

LINCK, M ;

CREUTZBURG, H ;

POSTLE, AD ;

ARNING, A ;

MARTINCARRERA, I ;

SEWING, KF .

ANALYTICAL BIOCHEMISTRY, 1994, 220 (01) :172-180

[4] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY SEPARATION ACID LIGHT-SCATTERING DETECTION OF PHOSPHOLIPIDS FROM COOKED BEEF [J].

CABONI, MF ;

MENOTTA, S ;

LERCKER, G .

JOURNAL OF CHROMATOGRAPHY A, 1994, 683 (01) :59-65

[5] A high-performance liquid chromatographic method for the analysis of lipids from lyophilized formulations [J].

Choudhari, KB ;

Jayanthi, S ;

Murty, RB ;

Matharu, RP .

JOURNAL OF CHROMATOGRAPHY A, 1996, 724 (1-2) :343-347

[6]

FOLCH J, 1957, J BIOL CHEM, V226, P497

[7] Purification of cholesterol-esterifying enzymes from rat liver cytosol by high-performance liquid chromatography [J].

Hradec, J ;

Franek, F ;

Dufek, P .

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS, 1996, 681 (01) :55-62

[8] SIMULTANEOUS DETERMINATION OF PHOSPHOLIPID CLASSES AND THE MAJOR MOLECULAR-SPECIES OF LECITHIN IN HUMAN AMNIOTIC-FLUID BY HPLC [J].

KLEIN, BH ;

DUDENHAUSEN, JW .

JOURNAL OF LIQUID CHROMATOGRAPHY, 1994, 17 (05) :981-998

[9]

ODA PJ, 1990, LIPIDS, V26, P756

[10]

PATTON GM, 1982, J LIPID RES, V23, P190

← 1 2 →