Evaluation of a miniaturized thermal biosensor for the determination of glucose in whole blood

被引:32
作者
Harborn, U
Xie, B
Venkatesh, R
Danielsson, B
机构
[1] Univ Lund, Ctr Chem, Dept Pure & Appl Biochem, S-22100 Lund, Sweden
[2] Voluntary Hlth Serv, PROMED, Madras 600113, Tamil Nadu, India
关键词
flow injection analysis; thermal; calorimetric; biosensor; glucose oxidase; calibration aspects; diabetes; enzyme thermistor;
D O I
10.1016/S0009-8981(97)00151-4
中图分类号
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号
1001 ;
摘要
A miniaturized thermal biosensor has been evaluated as part of a flow-injection analysis system for the determination of glucose in whole blood. Glucose was determined by measuring the heat evolved when samples containing glucose passed through a small column with immobilized glucose oxidase and catalase. Samples of whole blood (1 mu l) can be measured directly, without any pretreatment. The correlation in the response between the thermal biosensor, the Reflolux S meter (Boehringer Mannheim), the Granutest 100 glucose test kit (Merck Diagnostica) and the Ektachem (Kodak) instrument was evaluated. The influence of the hematocrit value and of possible interferences is reported. The correlation measurements show that the thermal biosensor calibrated with aqueous glucose standards generally gives lower values on blood glucose than the reference methods calibrated for serum or blood measurements. Mean negative biases range from 0.53 to 1.16 mmol/l. Differences in sample treatment clearly complicate comparisons and the proper choice of reference method. There was no influence from substances such as ascorbic acid (0.11 mmol/l), uric acid (0.48 mmol/l), urea (4.3 mmol/l) and acetaminophen (0.17 mmol/l) on the response to 5 mmol/l glucose. The hematocrit value does not influence the glucose determination, for hematocrit values of between 13 and 53%. (C) 1997 Elsevier Science B.V.
引用
收藏
页码:225 / 237
页数:13
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