Cloning and expression of fatty acids biosynthesis key enzymes from sunflower (Helianthus annuus L.) in Escherichia coli

被引：18

作者：

Serrano-Vega, MJ ^{[1
]}

Venegas-Calerón, M ^{[1
]}

Garcés, R ^{[1
]}

Martínez-Force, E ^{[1
]}

机构：

[1] CSIC, Inst Grasa & Derivados, E-41012 Seville, Spain

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2003年 / 786卷 / 1-2期

关键词：

cloning; expression; Escherichia coli; purification; fatty acids; stearoyl-acyl carrier protein desaturase;

D O I：

10.1016/S1570-0232(02)00767-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

To further characterize the stearoyl-acyl carrier protein (ACP) desaturase (EC 1.14.99.6) and the acyl-ACP thioesterase FatB (EC 3.1.2.14) activities from sunflower seeds, we cloned, sequenced and expressed the recombinant genes in Escherichia coli. We obtained two partially purified proteins, His-SAD and His-FATB, each of about 45 000 Da. The expression of either proteins produced changes in the E. coli fatty acid profile indicating the functionality of the recombinant proteins. While the expression of His-SAD produced an effect similar to that produced by overexpression of the fabA gene, responsible for the fatty acid desaturation in E. coli, the expression of Ms-FATB gave rise to an unbalance between unsaturated fatty acids and a toxic effect in E. coli. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：221 / 228

页数：8

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