O-glycoside orientation is an essential aspect of base J recognition by the kinetoplastid DNA-binding protein JBP1

被引：22

作者：

Grover, Rajesh K.

Pond, Stephanie J. K.

Cui, Qizhi

Subramaniam, Prem

Case, David A.

Millar, David P.

Wentworth, Paul, Jr.

机构：

[1] Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA

[2] Scripps Res Inst, Skaggs Inst Chem Biol, La Jolla, CA 92037 USA

[3] Univ Oxford, Dept Biochem, Scripps Oxford Lab, Oxford Glycobiol Inst, Oxford OX1 3QU, England

[4] Scripps Res Inst, Dept Mol Biol, La Jolla, CA 92037 USA

[5] Scripps Res Inst Florida, Dept Infectol, Jupiter, FL 32458 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2007年 / 46卷 / 16期

关键词：

DNA; glycosides; molecular dynamics; molecular recognition;

D O I：

10.1002/anie.200604635

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A binding edge? Comparison of dissociation constants and molecular-dynamics snapshots of a panel of synthetic telomeric double-stranded DNA sequences containing HMdU O-glycosides has revealed that the DNA-binding protein of kinetoplastids, such as Leishmania and Trypanosoma, JBP1, recognizes a critical edge-on conformation of the pyranosyl ring of base J. If this conformation is perturbed, JBP1 binding is dramatically reduced.(Chemical Equation Presented). © 2007 Wiley-VCH Verlag GmbH & Co. KGaA.

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页码：2839 / 2843

页数：5

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