Development of a tightly regulated U6 promoter for shRNA expression

被引：31

作者：

Lin, XY ^{[1
]}

Yang, JG ^{[1
]}

Chen, J ^{[1
]}

Gunasekera, A ^{[1
]}

Fesik, SW ^{[1
]}

Shen, Y ^{[1
]}

机构：

[1] Abbott Labs, Global Pharmaceut Res & Dev, Canc Res, Abbott Pk, IL 60064 USA

来源：

FEBS LETTERS | 2004年 / 577卷 / 03期

关键词：

short hairpin RNA; small interfering RNA; tetracycline-responsive; U6; promoter; tight regulation;

D O I：

10.1016/j.febslet.2004.10.033

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Short hairpin RNAs (shRNAs) have been used to achieve stable target knockdown in a variety of biological systems. Here, we report the development of a tightly regulated tetracycline-responsive human U6 promoter for shRNA expression. By engineering two copies of the tet operators flanking the TATA box of the human U6 promoter, we created a U6 promoter derivative (202) that exhibited much lower basal transcriptional activity compared with recently reported inducible pol III dependent promoters. As a consequence of its tighter regulation, the 202 system greatly improved the success rate in making inducible knockdown cell lines. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：376 / 380

页数：5

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