BipA:: a tyrosine-phosphorylated GTPase that mediates interactions between enteropathogenic Escherichia coli (EPEC) and epithelial cells

被引:83
作者
Farris, M [1 ]
Grant, A [1 ]
Richardson, TB [1 ]
O'Connor, CD [1 ]
机构
[1] Univ Southampton, Dept Biochem, Southampton SO16 7PX, Hants, England
基金
英国惠康基金;
关键词
D O I
10.1046/j.1365-2958.1998.00793.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We report the functional characterization of BipA, a GTPase that undergoes tyrosine phosphorylation in an enteropathogenic Escherichia coli (EPEC) strain. BipA(-) mutants adhere to cultured epithelial cells but fail to trigger the characteristic cytoskeletal rearrangements found in cells infected with wild-type EPEC. In contrast, increased expression of BipA enhances actin remodelling and results in the hyperformation of pseudopods. BipA appears to be the first example of a new class of virulence regulator, as it also controls flagella-mediated cell motility and resistance to the antibacterial effects of a human host defence protein. Its striking sequence similarity to ribosome-binding elongation factors suggests that it uses a novel mechanism to modulate gene expression.
引用
收藏
页码:265 / 279
页数:15
相关论文
共 52 条
  • [1] Rho-dependent membrane folding causes Shigella entry into epithelial cells
    Adam, T
    Giry, M
    Boquet, P
    Sansonetti, P
    [J]. EMBO JOURNAL, 1996, 15 (13) : 3315 - 3321
  • [2] Gapped BLAST and PSI-BLAST: a new generation of protein database search programs
    Altschul, SF
    Madden, TL
    Schaffer, AA
    Zhang, JH
    Zhang, Z
    Miller, W
    Lipman, DJ
    [J]. NUCLEIC ACIDS RESEARCH, 1997, 25 (17) : 3389 - 3402
  • [3] PLASMID-MEDIATED ADHESION IN ENTEROPATHOGENIC ESCHERICHIA-COLI
    BALDINI, MM
    KAPER, JB
    LEVINE, MM
    CANDY, DCA
    MOON, HW
    [J]. JOURNAL OF PEDIATRIC GASTROENTEROLOGY AND NUTRITION, 1983, 2 (03) : 534 - 538
  • [4] BOURNE HR, 1991, NATURE, V349, P117, DOI 10.1038/349117a0
  • [5] Requirement of CDC42 for Salmonella-induced cytoskeletal and nuclear responses
    Chen, LM
    Hobbie, S
    Galan, JE
    [J]. SCIENCE, 1996, 274 (5295) : 2115 - 2118
  • [6] ROLE OF THE EAEA GENE IN EXPERIMENTAL ENTEROPATHOGENIC ESCHERICHIA-COLI INFECTION
    DONNENBERG, MS
    TACKET, CO
    JAMES, SP
    LOSONSKY, G
    NATARO, JP
    WASSERMAN, SS
    KAPER, JB
    LEVINE, MM
    [J]. JOURNAL OF CLINICAL INVESTIGATION, 1993, 92 (03) : 1412 - 1417
  • [7] ENTEROPATHOGENIC ESCHERICHIA-COLI
    DONNENBERG, MS
    KAPER, JB
    [J]. INFECTION AND IMMUNITY, 1992, 60 (10) : 3953 - 3961
  • [8] CONSTRUCTION OF AN EAE DELETION MUTANT OF ENTEROPATHOGENIC ESCHERICHIA-COLI BY USING A POSITIVE-SELECTION SUICIDE VECTOR
    DONNENBERG, MS
    KAPER, JB
    [J]. INFECTION AND IMMUNITY, 1991, 59 (12) : 4310 - 4317
  • [9] Autophosphorylation of a bacterial protein at tyrosine
    Duclos, B
    Grangeasse, C
    Vaganay, E
    Riberty, M
    Cozzone, AJ
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 1996, 259 (05) : 891 - 895
  • [10] SIGNAL-TRANSDUCTION IN HUMAN EPITHELIAL-CELLS INFECTED WITH ATTACHING AND EFFACING ESCHERICHIA-COLI IN-VITRO
    DYTOC, M
    FEDORKO, L
    SHERMAN, PM
    [J]. GASTROENTEROLOGY, 1994, 106 (05) : 1150 - 1161