Polyploidy in tomato roots as affected by arbuscular mycorrhizal colonization

被引:24
作者
Berta, G
Fusconi, A
Sampò, S
Lingua, G
Perticone, S
Repetto, O
机构
[1] Univ Piemonte Orientale, Dipartimento Sci & Tecnol Avanzate, I-15100 Alessandria, Italy
[2] Univ Turin, Dipartimento Biol Vegetale, I-10125 Turin, Italy
关键词
flow and static cytometry; Glomus mosseae; Lycopersicon esculentum; polyploidization; respiratory activity;
D O I
10.1023/A:1026468810886
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Nuclear changes in roots of tomato (Lycopersicon esculentum), a plant with a small genome, during the establishment of arbuscular mycorrhizal (AM) colonization were studied using light and electron microscopy, as well as flow and static cytometry. Nuclei of mycorrhizal root cortex cells were larger and had more decondensed chromatin than those of controls. Significant ploidy distribution differences were observed between nuclei of AM colonized and control roots, and a strong correlation between nuclear polyploidization and AM colonization was found. Polyploidization and decondensation are usually associated with high metabolic activity. The metabolic activity of mycorrhizal root cells, evaluated in this work as respiratory activity by using a cytochemical assay for succinate dehydrogenase combined with image analysis, increased in comparison to controls. The meaning of polyploidization is discussed in relation to the structural and metabolic modifications induced by mycorrhization.
引用
收藏
页码:37 / 44
页数:8
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