Activation of mitogen-activated protein kinase is involved in sphingosine 1-phosphate-stimulated interleukin-6 synthesis in osteoblasts

被引：39

作者：

Kozawa, O ^{[1
]}

Tokuda, H ^{[1
]}

Matsuno, H ^{[1
]}

Uematsu, T ^{[1
]}

机构：

[1] CHUBU NATL HOSP,NATL INST LONGEV SCI,DEPT INTERNAL MED,OBU,AICHI 474,JAPAN

来源：

FEBS LETTERS | 1997年 / 418卷 / 1-2期

关键词：

mitogen-activated protein kinase; sphingosine; 1-phosphate; interleukin-6;

D O I：

10.1016/S0014-5793(97)01366-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We previously showed that sphingosine 1-phosphate (SPP) acts as a second messenger for tumor necrosis factor alpha-induced interleukin-6 (IL-6) synthesis in osteoblast-like MC3T3-E1 cells, In the present study, we further investigated the mechanism of IL-6 synthesis induced by SPP in in MC3T3-E1 cells, SPP significantly induced p42/p44 mitogen-activated protein (MAP) kinase activity. PD98059, an inhibitor of MAP kinase kinase, suppressed SPP-induced IL-6 synthesis as well as SPP-induced MAP kinase activation, The patterns of both inhibitions were similar, TMB-8, an inhibitor of Ca2+ mobilization from intracellular Ca2+ stores, significantly suppressed the SPP-induced IL-6 synthesis, These results strongly suggest that SPP-induced IL-6 synthesis is mediated via p42/p44 MAP kinase activation in osteoblast-like cells and that the SPP-induced IL-6 synthesis is dependent on intracellular Ca2+ mobilization. (C) 1997 Federation of European Biochemical Societies.

引用

页码：149 / 151

页数：3

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