Sensitization of thermotolerant SCK cells to hyperthermia and freezing with reduction of intracellular pH: Implications for cryosurgery

Background and Objectives: During cryosurgery, cells frozen slowly at the outer part of the ice ball undergo severe dehydration and are subject to solute effects injury, which may be caused in part by protein denaturation. This study was undertaken to determine whether heat shock proteins (HSPs), the molecular chaperones that stabilize proteins against denaturation, have a protective effect on cells during slow freezing. In addition, we aimed to determine whether acidic conditions, similar to those found in many solid tumors, would effect this protection. Methods: SCK cells were frozen at 5degreesC/min to -10degreesC or -20degreesC before or after induction of thermotolerance, and at neutral or low pH conditions. Lethal damage was determined by clonogenics. Results: Clonogenic survival was decreased by 50% in thermotolerant cells frozen to -10degreesC after culture in acidic conditions ( pH 6.6) compared with non-thermotolerant cells cultured at neutral pH. Induction of thermotolerance alone or low pH alone did not significantly sensitize SCK cells to freezing. All treatment groups were equally susceptible to killing when frozen to -20degreesC. Conclusions: Our results show that induction of thermal tolerance does not protect SCK cells against subsequent freezing injury and that a low pH environment actually sensitizes these cells to freeze injury. (C) 2003 Wiley-Liss, Inc.