Progress in phage display: evolution of the technique and its applications

被引：145

作者：

Bratkovic, Tomaz ^{[1
]}

机构：

[1] Univ Ljubljana, Fac Pharm, Dept Pharmaceut Biol, Ljubljana 1000, Slovenia

来源：

CELLULAR AND MOLECULAR LIFE SCIENCES | 2010年 / 67卷 / 05期

关键词：

Phage display; Molecular evolution; Library screening; Library design; Selection strategy; IN-VIVO RECOMBINATION; ZINC-FINGER DOMAINS; COMBINATORIAL ANTIBODY LIBRARIES; MAJOR COAT PROTEIN; FILAMENTOUS PHAGE; HELPER PHAGE; SURFACE DISPLAY; BACTERIOPHAGE-LAMBDA; PEPTIDE LIBRARIES; HEREDITARY ANGIOEDEMA;

D O I：

10.1007/s00018-009-0192-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Phage display, the presentation of (poly)peptides as fusions to capsid proteins on the surface of bacterial viruses, celebrates its 25th birthday in 2010. The technique, coupled with in vitro selection, enables rapid identification and optimization of proteins based on their structural or functional properties. In the last two decades, it has advanced tremendously and has become widely accepted by the scientific community. This by no means exhaustive review aims to inform the reader of the key modifications in phage display. Novel display formats, innovative library designs and screening strategies are discussed. I will also briefly review some recent uses of the technology to illustrate its incredible versatility.

引用

页码：749 / 767

页数：19

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