Quantitative differences in intervertebral disc-matrix composition with age-related degeneration

被引：14

作者：

Murakami, Hideki ^{[2
,3
,4
]}

Yoon, Tim S. ^{[3
,4
]}

Attallah-Wasif, Emad S. ^{[3
,4
]}

Kraiwattanapong, Chaiwat ^{[3
,4
]}

Kikkawa, Ichiro ^{[3
,4
]}

Hutton, William C. ^{[1
,3
,4
]}

机构：

[1] Emory Orthopaed & Spine Ctr, Atlanta, GA 30329 USA

[2] Iwate Med Univ, Sch Med, Dept Orthopaed Surg, Morioka, Iwate 020, Japan

[3] Atlanta Vet Adm Med Ctr, Decatur, GA USA

[4] Emory Univ, Dept Orthopaed, Sch Med, Atlanta, GA USA

来源：

MEDICAL & BIOLOGICAL ENGINEERING & COMPUTING | 2010年 / 48卷 / 05期

关键词：

Intervertebral disc; Age; Water content; sGAG; DNA; Aggrecan; Type I collagen; Type II collagen; Real-time PCR; COLLAGEN TYPES; PROTEOGLYCANS; PROTEIN; SPINE; MODEL;

D O I：

10.1007/s11517-010-0586-1

中图分类号：

TP39 [计算机的应用];

学科分类号：

080201 [机械制造及其自动化];

摘要：

This study was carried out to determine the effect of age on the intervertebral disc, using a rabbit model. Anulus fibrosus and nucleus pulposus tissue from New Zealand white rabbits aged 3 years old (old rabbits) and 6 months old (young rabbits) were used. The water content, the proteoglycan, the DNA content, and the mRNA levels of aggrecan, type I collagen, and type II collagen were all measured for each sample. Water, proteoglycan, DNA, and the mRNA levels of aggrecan and type II collagen were all greater in the nucleus pulposus of the young rabbits as compared to the old. For the anulus fibrosus, the difference between young and old is less marked with only proteoglycan and DNA being greater in the young disc as compared to the old. Clearly, according to our results, it is the nucleus pulposus that suffers the brunt of the changes with age.

引用

页码：469 / 474

页数：6

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