Positional cloning and gene identification

After genetic mapping and physical representation of a particular genomic region containing the gene underlying a particular Mendelian trait, a successful positional cloning strategy depends on the efficient detection and analysis of genes in the critical interval. Several gene detection strategies are presently available to compile an inventory of genes from large genomic regions. Here, the principle of these methods is briefly reviewed and their relative value for positional cloning projects compared. (C) 1998 Academic Press.