Evaluation of an ultrasensitive p24 antigen assay as a potential alternative to human immunodeficiency virus type 1 RNA viral load assay in resource-limited settings

被引：32

作者：

Respess, RA

Cachafeiro, A

Withum, D

Fiscus, SA

Newman, D

Branson, B

Varnier, OE

Lewis, K

Dondero, TJ

机构：

[1] Ctr Dis Control & Prevent, Global AIDS Program, Natl Ctr HIV,STD & TB Prevent, Atlanta, GA 30333 USA

[2] Univ N Carolina, Chapel Hill, NC 27515 USA

[3] Univ Genoa, I-16126 Genoa, Italy

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2005年 / 43卷 / 01期

关键词：

D O I：

10.1128/JCM.43.1.506-508.2005

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

An inexpensive enzyme-linked immunosorbent assay method for human immunodeficiency virus type I quantitation, ultrasensitive p24 antigen assay (Up24), was compared with RNA viral load assay (VL). Up24 had 100% sensitivity of detection at a viral load of greater than or equal to30,000, with sensitivity of 46.4% at a viral load of <30,000 (232 specimens from 65 seropositive subjects). The assay was highly reproducible, with excellent correlation between duplicates and among three laboratories.

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页码：506 / 508

页数：3

相关论文

共 16 条

[1] Simple monitoring of antiretroviral therapy with a signal-amplification-boosted HIV-1 p24 antigen assay with heat-denatured plasma [J].

Boni, J ;

Opravil, M ;

Tomasik, Z ;

Rothen, M ;

Bisset, L ;

Grob, PJ ;

Luthy, R ;

Schupbach, J .

AIDS, 1997, 11 (06) :F47-F52

[2] A new quantitative HIV load assay based on plasma virion reverse transcriptase activity for the different types, groups and subtypes [J].

Braun, J ;

Plantier, JC ;

Hellot, MF ;

Tuaillon, E ;

Gueudin, M ;

Damond, F ;

Malmsten, A ;

Corrigan, GE ;

Simon, F .

AIDS, 2003, 17 (03) :331-336

[3]

BredbergRaden U, 1996, J ACQ IMMUN DEF SYND, V12, P421

[4]

Bürgisser P, 2000, J ACQ IMMUN DEF SYND, V23, P138

[5] A novel and innovative quantitative kinetic software for virological colorimetric assays [J].

Giacomini, M ;

McDermott, JL ;

Giri, AA ;

Martini, I ;

Lillo, FB ;

Varnier, OE .

JOURNAL OF VIROLOGICAL METHODS, 1998, 73 (02) :201-209

[6] Comparison of an amplified enzyme-linked immunosorbent assay with procedures based on molecular biology for assessing human immunodeficiency virus type 1 viral load [J].

Goldschmidt, PL ;

Devillechabrolle, A ;

Ait-Arkoub, Z ;

Aubin, JT .

CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY, 1998, 5 (04) :513-518

[7] Human immunodeficiency virus type 1 p24 concentration measured by boosted ELISA of heat-denatured plasma correlates with decline in CD4 cells, progression to AIDS, and survival:: Comparison with viral RNA measurement. (vol 181, pg 1280, 2000) [J].

Ledergerber, B ;

Flepp, M ;

Böni, J .

JOURNAL OF INFECTIOUS DISEASES, 2000, 181 (04) :1521-1521

[8] Development of calibrated viral load standards for group M subtypes of human immunodeficiency virus type 1 and performance of an improved AMPLICOR HIV-1 MONITOR test with isolates of diverse subtypes [J].

Michael, NL ;

Herman, SA ;

Kwok, S ;

Dreyer, K ;

Wang, J ;

Christopherson, C ;

Spadoro, JP ;

Young, KKY ;

Polonis, V ;

McCutchan, FE ;

Carr, J ;

Mascola, JR ;

Jagodzinsi, LL ;

Robb, ML .

JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (08) :2557-2563

[9] Prospective evaluation of amplification-boosted ELISA for heat-denatured p24 antigen for diagnosis and monitoring of pediatric human immunodeficiency virus type 1 infection [J].

Nadal, D ;

Böni, J ;

Kind, C ;

Varnier, OE ;

Steiner, F ;

Tomasik, Z ;

Schüpbach, J .

JOURNAL OF INFECTIOUS DISEASES, 1999, 180 (04) :1089-1095

[10] Comparison of an assay using signal amplification of the heat-dissociated p24 antigen with the Roche Monitor human immunodeficiency virus RNA assay [J].

Pascual, A ;

Cachafeiro, A ;

Funk, ML ;

Fiscus, SA .

JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (07) :2472-2475