The sulfhydryl oxidase Erv1 is a substrate of the Mia40-dependent protein translocation pathway

被引：54

作者：

Terziyska, Nadia

Grumbt, Barbara

Bien, Melanie

Neupert, Walter

Herrmann, Johannes M.

Hell, Kai

机构：

[1] Univ Munich, Adolf Butenandt Inst Physiol Chem, D-81377 Munich, Germany

[2] Univ Kaiserslautern, D-67663 Kaiserslautern, Germany

来源：

FEBS LETTERS | 2007年 / 581卷 / 06期

关键词：

protein import; Mia40-import pathway; sulfhydryl oxidase Erv1; mitochondria; disulfide bond;

D O I：

10.1016/j.febslet.2007.02.014

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The thiol oxidase Erv1 and the redox-regulated receptor Mia40/Tim4O are components of a disulfide relay system which mediates import of proteins into the intermembrane space (IMS) of mitochondria. Here we report that ErvI requires Mia40 for its import into mitochondria. After passage across the translocase of the mitochondrial outer membrane Ervl interacts via disullide bonds with Mia40. Erv1 does not contain twin "CX3C" or twin "CX9C" motifs which are crucial for import of typical substrates of this pathway and it does not need two "CX2C" motifs for import into mitochondria. Thus, Erv1 represents an unusual type of substrate of the Mia40-dependent import pathway. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

收藏

页码：1098 / 1102

页数：5

相关论文

共 23 条

[1] Erv1 mediates the Mia40-dependent protein import pathway and provides a functional link to the respiratory chain by shuttling electrons to cytochrome c [J].

Allen, S ;

Balabanidou, V ;

Sideris, DP ;

Lisowsky, T ;

Tokatlidis, K .

JOURNAL OF MOLECULAR BIOLOGY, 2005, 353 (05) :937-944

[2] Juxtaposition of the two distal CX3C motifs via intrachain disulfide bonding is essential for the folding of Tim10 [J].

Allen, S ;

Lu, H ;

Thornton, D ;

Tokatlidis, K .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (40) :38505-38513

[3] Folding studies of Cox17 reveal an important interplay of cysteine oxidation and copper binding [J].

Arnesano, F ;

Balatri, E ;

Banci, L ;

Bertini, I ;

Winge, DR .

STRUCTURE, 2005, 13 (05) :713-722

[4] Purification, characterization, and localization of yeast Cox17p, a mitochondrial copper shuttle [J].

Beers, J ;

Glerum, DM ;

Tzagoloff, A .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (52) :33191-33196

[5] Essential role of Mia40 in import and assembly of mitochondrial intermembrane space proteins [J].

Chacinska, A ;

Pfannschmidt, S ;

Wiedemann, N ;

Kozjak, V ;

Szklarz, LKS ;

Schulze-Specking, A ;

Truscott, KN ;

Guiard, B ;

Meisinger, C ;

Pfanner, N .

EMBO JOURNAL, 2004, 23 (19) :3735-3746

[6] Multidomain flavin-dependent sulfhydryl oxidases [J].

Coppock, Donald L. ;

Thorpe, Colin .

ANTIOXIDANTS & REDOX SIGNALING, 2006, 8 (3-4) :300-311

[7] Functional cooperation and separation of translocators in protein import into mitochondria, the double-membrane bounded organelles [J].

Endo, T ;

Yamamoto, H ;

Esaki, M .

JOURNAL OF CELL SCIENCE, 2003, 116 (16) :3259-3267

[8] Novel mitochondrial intermembrane space proteins as substrates of the MIA import pathway [J].

Gabriel, Kipros ;

Milenkovic, Dusanka ;

Chacinska, Agnieszka ;

Mueller, Judith ;

Guiard, Bernard ;

Pfanner, Nikolaus ;

Meisinger, Chris .

JOURNAL OF MOLECULAR BIOLOGY, 2007, 365 (03) :612-620

[9] The actin-binding domain of spinophilin is necessary and sufficient for targeting to dendritic spines [J].

Grossman, SD ;

Hsieh-Wilson, LC ;

Allen, PB ;

Nairn, AC ;

Greengard, P .

NEUROMOLECULAR MEDICINE, 2002, 2 (01) :61-69

[10] Chopped, trapped or tacked-protein translocation into the IMS of mitochondria [J].

Herrmann, JM ;

Hell, K .

TRENDS IN BIOCHEMICAL SCIENCES, 2005, 30 (04) :205-211