New N-glycans in horseradish peroxidase

被引：35

作者：

Takahashi, N

Lee, KB

Nakagawa, H

Tsukamoto, Y

Masuda, K

Lee, YC

机构：

[1] Nakano Vinegar Co Ltd, GlycoLab, Handa, Aichi 475, Japan

[2] Meijo Univ, Tempaku Ku, Nagoya, Aichi 468, Japan

[3] Johns Hopkins Univ, Dept Biol, Baltimore, MD 21218 USA

来源：

ANALYTICAL BIOCHEMISTRY | 1998年 / 255卷 / 02期

关键词：

horseradish peroxidase; N-glycans; N-linked oligosaccharides; glycoamidases A;

D O I：

10.1006/abio.1997.2463

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe here the structures of several new N-glycan oligosaccharides from horseradish peroxidase (HRP). Glycopeptides from HRP were digested with glccoamidase A (from sweet almond) to release the N-glycans. The oligosaccharides were reductively aminated with 2-aminopyridine, and separated by highperformance liquid chromatography on octadecylsilyl- and amide-silica columns for two-dimensional mapping (Tomiya et al., Anal. Biochem. 171, 73-90, 1988). The N-glycans were also analyzed by electrospray ionization mass spectrometry. Two different lots of HRP showed a considerable difference in their N-glycan composition. (C) 1998 Academic Press.

引用

页码：183 / 187

页数：5

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