Production and purification of recombinant human α2C2 adrenergic receptor using Saccharomyces cerevisiae

被引：7

作者：

Kapat, A

Jaakola, VP

Heimo, H

Liitti, S

Heikinheimo, P

Glumoff, T

Goldman, A

机构：

[1] Univ Helsinki, Inst Biotechnol, FIN-00014 Helsinki, Finland

[2] Wallac Finland Oy, FIN-20101 Turku, Finland

[3] Univ Tromso, Fac Sci, Dept Chem, N-9037 Tromso, Norway

[4] Univ Oulu, Bioctr Oulu, FIN-90507 Oulu, Finland

[5] Univ Oulu, Dept Biochem, FIN-90507 Oulu, Finland

来源：

BIOSEPARATION | 2000年 / 9卷 / 03期

基金：

芬兰科学院;

关键词：

alpha(2)C2 adrenergic receptor; production; purification; recombinant; Saccharomyces cerevisiae;

D O I：

10.1023/A:1008150412294

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The objective is to generate milligram quantities of recombinant human alpha(2)C2 adrenergic receptor for X-ray crystallographic studies. It has been cloned in Saccharomyces cerevisiae, and the production level is at best about 13 pmol/mg of membrane protein, as estimated by radio-ligand binding assay. The receptor is solubilized with sucrose monolaurate followed by immunoaffinity purification and reconstitution into phospholipid vesicles. The efficiency of solubilization and immuno-purification are 60% and 91%, respectively.

引用

页码：167 / 172

页数：6

共 16 条

[1]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[2] SUBTYPES OF ALPHA-2-ADRENOCEPTORS - PHARMACOLOGICAL AND MOLECULAR BIOLOGICAL EVIDENCE CONVERGE [J].